{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:48:01Z","timestamp":1761155281434},"reference-count":31,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":10657,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1980,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Glycoproteins of <jats:italic>Trypanosoma congolense<\/jats:italic> have been detected on SDS-polyacrylamide gels using the Concanavalin A peroxidase technique. Using [<jats:sup>35<\/jats:sup>S]diazoniobenzenesulphonate as a marker for cell surface proteins it was possible to distinguish between internal glycoproteins and the surface coat proteins. On SDS-polyacrylamide gels Con A reacted with the surface coat proteins. Results obtained from Con A-induced agglutination of living trypanosomes indicated that sugars of the surface coat proteins were accessible to Con A. This was reinforced by the cytochemical visualization of Con A binding to the trypanosome surface. The results suggested that the surface coat protein contained \u03b1-linked <jats:sc>d<\/jats:sc>-mannosyl, <jats:sc>d<\/jats:sc>-glucosyl, or <jats:italic>N<\/jats:italic>-acetyl-<jats:sc>d<\/jats:sc>-glucosaminoyl residues, which are exposed exteriorly on the surface coat.<\/jats:p>","DOI":"10.1017\/s0031182000000561","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:49:10Z","timestamp":1244220550000},"page":"113-122","source":"Crossref","is-referenced-by-count":25,"title":["Evidence for concanavalin A binding sites on the surface coat of <i>Trypanosoma congolense<\/i>"],"prefix":"10.1017","volume":"80","author":[{"given":"P.","family":"Rautenberg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Reinwald","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H-J.","family":"Risse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000000561_ref013","first-page":"55","volume-title":"Concanavalin A as a Tool","author":"Goldstein","year":"1976"},{"key":"S0031182000000561_ref019","first-page":"549","article-title":"The binding properties of dimeric and tetrameric concanavalin A","volume":"248","author":"McKenzie","year":"1973","journal-title":"Journal of Biological Chemistry"},{"key":"S0031182000000561_ref024","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(79)90123-1"},{"key":"S0031182000000561_ref007","doi-asserted-by":"publisher","DOI":"10.1017\/S003118200004717X"},{"key":"S0031182000000561_ref022","doi-asserted-by":"publisher","DOI":"10.1515\/bchm2.1978.359.2.939"},{"key":"S0031182000000561_ref015","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1977.tb01020.x"},{"key":"S0031182000000561_ref021","first-page":"199","volume-title":"Ciba Fdn Symp.","volume":"20","author":"Raadt","year":"1974"},{"key":"S0031182000000561_ref031","doi-asserted-by":"publisher","DOI":"10.2307\/3277712"},{"key":"S0031182000000561_ref008","first-page":"413","volume-title":"Biochemistry of Parasites and Host\u2013Parasite Relationships","author":"Cross","year":"1976"},{"key":"S0031182000000561_ref014","first-page":"493","volume-title":"Biology of the Kinetoplastida","volume":"1","author":"Gray","year":"1976"},{"key":"S0031182000000561_ref025","doi-asserted-by":"publisher","DOI":"10.1038\/271654a0"},{"key":"S0031182000000561_ref012","first-page":"35","volume-title":"Advances in Experimental Medicine and Biology","volume":"55","author":"Goldstein","year":"1975"},{"key":"S0031182000000561_ref001","first-page":"761","article-title":"Affinit\u00e9 de la concanavaline A pour Trypanosoma equiperdum: applications \u00e0 l'isolement de la fraction glycoprot\u00e9ique sp\u00e9cifique du type antig\u00e9nique","volume":"127 C","author":"Baltz","year":"1976","journal-title":"Annales d'Immunologie (Inst. 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