{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T20:51:19Z","timestamp":1698353479498},"reference-count":25,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":8953,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1984,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Adult <jats:italic>Onchocerca volvulus<\/jats:italic> worms obtained by enzyme digestion from nodules of infected Mexicans were radio-isotope labelled by the chloramine-T or Bolton\u2013Hunter methods. No antigenic determinants were detected in extracts of worms labelled by the chloramine-T method but 3 antigens were detected in extracts of the Bolton\u2013Hunter labelled worms. Two were present in such small amounts that it was impractical to investigate them further, but a major component of mol. wt 20 kDa was purified by gel filtration and used in a serological survey of inhabitants of villages in Southern Mexico. Using the 20 kDa antigen, which is superficially located on both sexes of <jats:italic>O. volvulus<\/jats:italic>, sera from both non-endemic and endemic regions were analysed by radio-immunoprecipitation of this antigen. In Southern Mexico, the average sensitivity of the test was 92%, and the specificity 98%. Whilst the 20 kDa antigen did not detect antibodies in the sera of Trinidadians infected with <jats:italic>Wuncheria bancrofti<\/jats:italic> or <jats:italic>Mansonella ozzardi<\/jats:italic>, this antigen detected high levels of antibodies in Indians exposed to <jats:italic>W. bancrofti<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0031182000001323","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:26:48Z","timestamp":1244219208000},"page":"295-310","source":"Crossref","is-referenced-by-count":36,"title":["Identification of an antigen of <i>Onchocerca volvulus<\/i> of possible diagnostic use"],"prefix":"10.1017","volume":"89","author":[{"given":"M.","family":"Philipp","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"G\u00f3mez-Priego","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. M. E.","family":"Parkhouse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. W.","family":"Davies","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. W. T.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bridget M.","family":"Ogilvie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Beltr\u00e1n-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000001323_ref009","doi-asserted-by":"publisher","DOI":"10.2307\/3275923"},{"key":"S0031182000001323_ref001","first-page":"108","article-title":"Immunological diagnosis of human filariasis: Present possibilities, difficulties and limitations","volume":"31","author":"Ambroise-Thomas","year":"1974","journal-title":"Acta Tropica"},{"key":"S0031182000001323_ref017","doi-asserted-by":"publisher","DOI":"10.1038\/287538a0"},{"key":"S0031182000001323_ref008","first-page":"382","article-title":"Immunoglobulins and complement in sera from patients with various parasitic infections","volume":"59","author":"Higashi","year":"1971","journal-title":"Indian Journal of Medical Research"},{"key":"S0031182000001323_ref021","first-page":"428","article-title":"Isolation of living adult Onchocerca volvulus from nodules","volume":"28","author":"Schulz-Key","year":"1977","journal-title":"Tropenmedizin und Parasitologie"},{"key":"S0031182000001323_ref013","first-page":"569","article-title":"Application des extraits antig\u00e9niques d'Ascaris suum au diagnostic des filarioses en double diffusion. 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Levels of IgG, IgM or IgD in different protozoal and helminthic infections in man","volume":"64","author":"Cappuccinelli","year":"1971","journal-title":"Giornale di Batteriologia e Virol"},{"key":"S0031182000001323_ref011","first-page":"454","article-title":"Cross-reacting antigens among some Filariae and other nematodes","volume":"25","author":"Neppert","year":"1974","journal-title":"Tropenmedizin und Parasitologie"},{"key":"S0031182000001323_ref003","first-page":"21","article-title":"Serum immunoglobulin levels in five villages of the republic of Chad and in onchocerciasis patients with and without microfilaremia","volume":"24","author":"Buck","year":"1973","journal-title":"Tropenmedizin und Parasitologie"},{"key":"S0031182000001323_ref012","first-page":"36","article-title":"Seroepidemiological aspects of onchocerciasis in Liberia, West Africa","volume":"29","author":"Neppert","year":"1978","journal-title":"Tropenmedizin und Parasitologie"},{"key":"S0031182000001323_ref022","first-page":"107","article-title":"A three year follow up of ocular onchocerciasis in an area of vector control","volume":"58","author":"Thylefors","year":"1980","journal-title":"Bulletin of the World Health Organization"},{"key":"S0031182000001323_ref023","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(81)90328-8"},{"key":"S0031182000001323_ref025","first-page":"129","article-title":"IgE antibodies are more species-specific than IgG antibodies in human onchocerciasis and lymphatic filariasis","volume":"45","author":"Weiss","year":"1982","journal-title":"Immunology"},{"key":"S0031182000001323_ref020","volume-title":"Human Filariasis: a Global Survey of Epidemiology and Control","author":"Sasa","year":"1976"},{"key":"S0031182000001323_ref024","first-page":"353","article-title":"IgE antibodies in human onchocerciasis. 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