{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T23:48:31Z","timestamp":1793058511377,"version":"4.5.0"},"reference-count":9,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":14858,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1968,8]]},"abstract":"<jats:p>A method of breeding and maintaining <jats:italic>L. natalensis<\/jats:italic> cultures in the laboratory is described.<\/jats:p><jats:p>The infection of snails with <jats:italic>F. gigantica<\/jats:italic> miracidia and the collection of metacercariae is discussed. The mean number of metacercariae arising from cercariae produced by each snail was 652\u00b76, over a period of 8\u00b72 days.<\/jats:p><jats:p>It is shown that after 3 months storage <jats:italic>F. gigantica<\/jats:italic> metacercariae are still viable when excysted <jats:italic>in vitro<\/jats:italic> by using artificial pepsin and trypsin enzymes.<\/jats:p><jats:p>The author wishes to express his thanks to Dr G. M. Urquhart of the Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Veterinary Hospital, Bearsden Road, Bearsden, Glasgow, who has read through this paper and made the necessary corrections.<\/jats:p>","DOI":"10.1017\/s0031182000028948","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T17:17:23Z","timestamp":1244222243000},"page":"653-656","source":"Crossref","is-referenced-by-count":8,"title":["<i>Lymnaea natalensis<\/i> laboratory culture and production of <i>Fasciola gigantica<\/i> metacercariae"],"prefix":"10.1017","volume":"58","author":[{"given":"P. K.","family":"Bitakaramire","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000028948_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(54)90016-8"},{"key":"S0031182000028948_ref007","doi-asserted-by":"publisher","DOI":"10.1038\/161894a0"},{"key":"S0031182000028948_ref005","first-page":"1","article-title":"The freshwater mollusks of Uganda and adjacent territories","volume":"32","author":"Mandahl-Barth","year":"1954","journal-title":"Annls Mus. r. Congo beige"},{"key":"S0031182000028948_ref006","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1951.11685473"},{"key":"S0031182000028948_ref004","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1965.11686275"},{"key":"S0031182000028948_ref002","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000074321"},{"key":"S0031182000028948_ref003","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1959.11685919"},{"key":"S0031182000028948_ref001","first-page":"338","article-title":"Observations on the succession of redial generations of Fasciola gigantica Cobbold in a snail host","volume":"7","author":"Dinnik","year":"1956","journal-title":"Tropenmed. u. Parasit."},{"key":"S0031182000028948_ref009","first-page":"895","article-title":"A rapid method for determining the viability of Fasciola hepatica metacercariae","volume":"21","author":"Wikerhauser","year":"1960","journal-title":"Am. J. vet. Res."}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000028948","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T21:00:44Z","timestamp":1559422844000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000028948\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,8]]},"references-count":9,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1968,8]]}},"alternative-id":["S0031182000028948"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000028948","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1968,8]]}}}