{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T07:57:07Z","timestamp":1761292627501},"reference-count":26,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":10291,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1981,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Freshly excysted<jats:italic>Fasciola hepatica<\/jats:italic>possess an outer glycocalyx which stimulates an antibody response in cattle infected with<jats:italic>F. hepatica<\/jats:italic>metacercariae. Sera from animals receiving either a single or a double infection were examined for levels of IgM, IgG<jats:sub>1<\/jats:sub>and IgG<jats:sub>2<\/jats:sub>specific for the outer glycocalyx of intact live juvenile<jats:italic>F. hepatica<\/jats:italic>. High levels of specific IgG<jats:sub>1<\/jats:sub>were found to predominate. Using positive sera in an indirect fluorescent antibody assay, the bovine immunoglobulins labelled the outer glycocalyx in a characteristic reticulated pattern. On incubation at 37 \u00b0C this pattern rapidly broke up into discrete clumps and progressed until the entire antibody\u2013outer glycocalyx complex was shed. The shedding phenomenon was shown to be continuous and, by using preparations of Fab fragments, was not due to the bivalent binding of the sensitizing antibody.<\/jats:p>","DOI":"10.1017\/s0031182000041809","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:44:44Z","timestamp":1244220284000},"page":"1-10","source":"Crossref","is-referenced-by-count":29,"title":["The interaction<i>in vitro<\/i>between bovine immunoglobulin and juvenile<i>Fasciola hepatica<\/i>"],"prefix":"10.1017","volume":"82","author":[{"given":"W. P. H.","family":"Duffus","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Franks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000041809_ref011","first-page":"347","article-title":"Initial studies on the properties of a bovine lymphoid cell culture infected with Theileria parva","volume":"34","author":"Duffus","year":"1978","journal-title":"Clinical and Experimental Immunology"},{"key":"S0031182000041809_ref014","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/S0034-5288(18)33259-4","article-title":"Antibody response of calves to a single infection of Fasciola gigantica determined by an indirect fluorescent antibody technique","volume":"22","author":"Hanna","year":"1977","journal-title":"Research in Veterinary Science"},{"key":"S0031182000041809_ref008","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/S0034-5288(18)33993-6","article-title":"Evidence of an acquired resistance in calves to a single experimental infection of Fasciola hepatica","volume":"13","author":"Doyle","year":"1972","journal-title":"Research in Veterinary Science"},{"key":"S0031182000041809_ref022","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1979.tb15163.x"},{"key":"S0031182000041809_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(73)90004-0"},{"key":"S0031182000041809_ref012","first-page":"181","article-title":"Acquisition of human blood group antigens by Schistosoma mansoni","volume":"26","author":"Goldring","year":"1976","journal-title":"Clinical and Experimental Immunology"},{"key":"S0031182000041809_ref015","unstructured":"El Harith A. (1977). Observations on the influence of the intermediate host in experimental fascioliasis. 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