{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T12:20:57Z","timestamp":1698841257243},"reference-count":36,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":12241,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1975,10]]},"abstract":"<jats:p>RNA extracted from purified parasites of<jats:italic>Plasmodium knowlesi<\/jats:italic>was fractionated using agarose gel electrophoresis. Preparations from parasites grown both<jats:italic>in vivo<\/jats:italic>and<jats:italic>in vitro<\/jats:italic>contained species of RNA with sedimentation coefficients of 4\u00b70S, 5\u00b70S, 16\u00b76S, 24\u00b72S, 31\u00b74S, 38\u00b70S and 48\u00b73S. There was less RNA present in parasites grown<jats:italic>in vitro<\/jats:italic>than the equivalent stage parasites grown<jats:italic>in vivo<\/jats:italic>but the proportional amounts of the various species of RNA was similar in both cases. It is suggested that the 24\u00b72S and 16\u00b76S species of RNA are ribosomal and that the high molecular weight 31\u00b74S, 38\u00b70S and 48\u00b70S species are ribosomal precursors. Ribosomal RNA synthesis occurs throughout the cell cycle during growth from the ring to the schizont stage; maximum incorporation of [H<jats:sup>3<\/jats:sup>]-adenosine occurs at the late trophozoite stage before nuclear division.<\/jats:p>","DOI":"10.1017\/s0031182000046655","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:57:35Z","timestamp":1244221055000},"page":"199-209","source":"Crossref","is-referenced-by-count":13,"title":["Ribonucleic acid synthesis in<i>Plasmodium knowlesi<\/i>maintained both<i>in vivo<\/i>and<i>in vitro<\/i>"],"prefix":"10.1017","volume":"71","author":[{"given":"P. I.","family":"Trigg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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