{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T08:41:43Z","timestamp":1710405703424},"reference-count":40,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":11267,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1978,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The freeze fracture technique has been used to quantify changes in the integral components of the double outer membrane of<jats:italic>Schistosoma mansoni<\/jats:italic>during the 6-week period of development within the mouse. The intramembraneous particle (IMP) density on the P<jats:sub>1<\/jats:sub>face begins to rise Within 6 h of host penetration, reaches a maximum at day 4 and then falls rapidly after day 9, so that it is at a low level between 3 and 6 weeks. The E1 face IMP density follows the same course as that of the P1 face except that maximum particle density is recorded on day 1 and the counts begin to fall on day 5. The IMP density on the P<jats:sub>1<\/jats:sub>face remains at a consistently low level throughout development. The E<jats:sub>2<\/jats:sub>face IMP density rises gradually to a peak at day 4, when the parasites have migrated to the lungs, and remains thereafter at a similar level, so that by 6 weeks the E<jats:sub>2<\/jats:sub>face has a higher IMP density than the other three fracture faces. The E<jats:sub>2<\/jats:sub>face IMP show a marked increase in size on day 4. Morphological studies indicate that a different type of inclusion body makes a transient appearance in the tegument of the lung worms, and immunocytochemical techniques show the lung worms to be nonimmunogenic. It is suggested, therefore, that the E<jats:sub>2<\/jats:sub>face IMP may represent complexes of parasite antigens and acquired host antigens. The tegumental membranes of cultured specimens have also been examined by freeze fracturing and the IMP densities compared with those obtained from<jats:italic>in vivo<\/jats:italic>parasites; the cultured schistosomula have a lower E<jats:sub>2<\/jats:sub>face particle density than the<jats:italic>in vivo<\/jats:italic>specimens.<\/jats:p>","DOI":"10.1017\/s0031182000048204","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T12:03:43Z","timestamp":1244203423000},"page":"327-348","source":"Crossref","is-referenced-by-count":48,"title":["A freeze fracture study of the developing tegumental outer membrane of<i>Schistosoma mansoni<\/i>"],"prefix":"10.1017","volume":"76","author":[{"given":"Diane J.","family":"McLaren","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. J.","family":"Hockley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O. L.","family":"Goldring","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. J.","family":"Hammond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000048204_ref031","doi-asserted-by":"publisher","DOI":"10.1021\/bi00735a023"},{"key":"S0031182000048204_ref001","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.71.3.876"},{"key":"S0031182000048204_ref005","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1242\/jcs.21.3.437","article-title":"A comparison of membrane fracture faces of fixed and unfixed glycerinated tissue","volume":"21","author":"Breathnach","year":"1976","journal-title":"Journal of Cell Science"},{"key":"S0031182000048204_ref035","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1969.0007"},{"key":"S0031182000048204_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(73)90004-0"},{"key":"S0031182000048204_ref008","doi-asserted-by":"publisher","DOI":"10.1038\/232653a0"},{"key":"S0031182000048204_ref014","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.72.3.667"},{"key":"S0031182000048204_ref024","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1111\/j.1365-2818.1965.tb02136.x","article-title":"Distortion in dimensions produced by shadowing for electron microscopy","volume":"84","author":"Misra","year":"1966","journal-title":"Journal of the Royal Microscopical Society"},{"key":"S0031182000048204_ref038","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.45.3.649"},{"key":"S0031182000048204_ref027","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.63.1.180"},{"key":"S0031182000048204_ref004","doi-asserted-by":"publisher","DOI":"10.1126\/science.1166299"},{"key":"S0031182000048204_ref032","unstructured":"Sher A. (1977). Immunity and immune evasion in schistosomiasis. In Proceedings of the Brook Lodge Symposium. Escape from Immune Surveillance, an Interpretative Survey of the Interface between Immune Mechanisms and Disease.(N.I.A.I.D. Sponsored Symposium)(in the Press)."},{"key":"S0031182000048204_ref028","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.45.3.598"},{"key":"S0031182000048204_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(75)90020-8"},{"key":"S0031182000048204_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(77)90083-9"},{"key":"S0031182000048204_ref017","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.9.2617"},{"key":"S0031182000048204_ref034","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000086248"},{"key":"S0031182000048204_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(65)90037-8"},{"key":"S0031182000048204_ref029","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.68.3.724"},{"key":"S0031182000048204_ref039","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000045777"},{"key":"S0031182000048204_ref019","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.6.1445"},{"key":"S0031182000048204_ref010","doi-asserted-by":"publisher","DOI":"10.1126\/science.179.4069.190"},{"key":"S0031182000048204_ref025","first-page":"449","volume-title":"Proceedings of the 7th International Congress of Electron Microscopy, Grenoble","volume":"1","author":"M\u00fchlethaler","year":"1970"},{"key":"S0031182000048204_ref033","first-page":"357","article-title":"Partial isolation of a membrane antigen which induces the formation of antibodies lethal to schistosornes cultured in vitro","volume":"18","author":"Seer","year":"1974","journal-title":"Clinical and Experimental Immunology"},{"key":"S0031182000048204_ref030","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1977.tb01492.x"},{"key":"S0031182000048204_ref037","first-page":"115","volume-title":"Advances in Parasitology","volume":"12","author":"Stirewalt","year":"1974"},{"key":"S0031182000048204_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(72)90036-7"},{"key":"S0031182000048204_ref009","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.3.648"},{"key":"S0031182000048204_ref036","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.7.2481"},{"key":"S0031182000048204_ref040","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.11.3891"},{"key":"S0031182000048204_ref022","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000046850"},{"key":"S0031182000048204_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(77)90010-8"},{"key":"S0031182000048204_ref026","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1975.tb04022.x"},{"key":"S0031182000048204_ref023","doi-asserted-by":"publisher","DOI":"10.1038\/269147a0"},{"key":"S0031182000048204_ref011","first-page":"181","article-title":"Acquisition of human blood group antigens by Schistosoma mansoni","volume":"26","author":"Goldring","year":"1976","journal-title":"Clinical and Experimental Immunology"},{"key":"S0031182000048204_ref020","doi-asserted-by":"publisher","DOI":"10.1177\/17.9.563"},{"key":"S0031182000048204_ref021","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000048873"},{"key":"S0031182000048204_ref003","first-page":"1","article-title":"Membrane structure","author":"Branton","year":"1972","journal-title":"Protoplasmatologia II\/E\/I"},{"key":"S0031182000048204_ref018","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000044231"},{"key":"S0031182000048204_ref002","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.73.2.400"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000048204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T08:24:26Z","timestamp":1710404666000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000048204\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,6]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1978,6]]}},"alternative-id":["S0031182000048204"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000048204","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,6]]}}}