{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T02:59:49Z","timestamp":1774666789469,"version":"3.50.1"},"reference-count":17,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":9502,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1983,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>In this study, we have developed a reliable radioimmunoassay (RIA) for<jats:italic>Ascaris suum<\/jats:italic>protein (Asp) and have measured the serum concentration of Asp in control subjects and in patients with helminthiasis and a variety of gastrointestinal diseases. The cross-reactivities of Asp antibody with other helminth worm antigens were also measured by RIA.<jats:italic>Ascaris lumbricoides<\/jats:italic>contained approximately the same amount of Asp antigenic substance as did<jats:italic>Ascaris suum. Toxocara canis<\/jats:italic>had a high concentration of a substance partially cross-reactive with Asp. Small amounts of substance cross-reacting with Asp were also exhibited by Anisakis larvae,<jats:italic>Schistosoma japonicum<\/jats:italic>and<jats:italic>Taenia saginata<\/jats:italic>. However, no cross-reactivities of<jats:italic>Fasciola hepatica<\/jats:italic>and<jats:italic>Paragonimus westermani<\/jats:italic>with Asp were observed in this study. Almost all the serum levels of Asp from normal control subjects were below the sensitivity of this RIA (10 ng\/ml). High concentrations of Asp were observed in sera from patients with ascariasis (64.5\u00b118.8 ng\/ml: mean \u00b1 S.E.), anisakiasis (75.2\u00b128.0 ng\/ml), toxocariasis (78.4\u00b131.3 ng\/ml) and schistosomiasis japonica (14.3\u00b13.4 ng\/ml). Asp levels in sera from patients with other helminthiasis were not significantly high. From these findings, it was suggested that RIA for Asp may not be specific for diagnosis of ascariasis, but might be a suitable screening method for patients with some kinds of helminthiasis. Interestingly, high concentrations of Asp were also observed in sera from patients with ulcerative colitis (24.8\u00b12.5 ng\/ml) and Crohn's disease (19.8\u00b15.2 ng\/ml), but Asp levels from 7 other gastrointestinal diseases seemed not to be significantly high. These findings remain unexplained but may derive from cross-reactivities between helminthic and gut mucosal antigens.<\/jats:p>","DOI":"10.1017\/s0031182000050459","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:35:36Z","timestamp":1244219736000},"page":"291-300","source":"Crossref","is-referenced-by-count":16,"title":["The measurement of<i>Ascaris suum<\/i>protein by radioimmunoassay in sera from patients with helminthiasis and with gastrointestinal diseases"],"prefix":"10.1017","volume":"86","author":[{"given":"K.","family":"Tanaka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Kawamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Tohgi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"TsujI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Miyachi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Miyoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000050459_ref016","first-page":"81","volume-title":"In Inflammatory Bowel Diseases","author":"Watson","year":"1975"},{"key":"S0031182000050459_ref015","doi-asserted-by":"publisher","DOI":"10.1159\/000231912"},{"key":"S0031182000050459_ref012","doi-asserted-by":"publisher","DOI":"10.1007\/BF02239587"},{"key":"S0031182000050459_ref011","first-page":"843","volume-title":"Gastroenterology","volume":"58","author":"Shorter","year":"1970"},{"key":"S0031182000050459_ref014","first-page":"180","volume-title":"In Proceedings of the SE AM EO\/Tropical Medicine and Technical Meeting","author":"Tsuji","year":"1974"},{"key":"S0031182000050459_ref010","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/S0016-5085(69)80129-0","article-title":"Further studies of in vitro cytotoxicity of lymphocytes from patients with ulcerative and granulomatous colitis for allogeneic colonic epithelial cells, including the effects of colectomy","volume":"56","author":"Shorter","year":"1969","journal-title":"Gastroenterology"},{"key":"S0031182000050459_ref007","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-34-1-23"},{"key":"S0031182000050459_ref003","first-page":"798","article-title":"Les antig\u00e8ns de Schistosoma mansoni. II. Etude immuno\u00e9lectrophor\u00e9tique compar\u00e9e de divers stades larvaires et des adultes des deux sexes. Aspects immunologiques des relations host-parasite de la cercaire et de l'adulte de S. mansoni","volume":"105","author":"Capron","year":"1965","journal-title":"Annates de l'lnstitut Pasteur"},{"key":"S0031182000050459_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/0019-2791(73)90185-7"},{"key":"S0031182000050459_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(69)90383-2"},{"key":"S0031182000050459_ref002","first-page":"3103","article-title":"Possibilit\u00e9s nouvelles dans le diagnostic immunologique de la distomatose humaine \u00e0 Fasciola hepatica. Mise en \u00e9vidence d'anticorps seriques par immuno\u00e9lectrophor\u00e9se","volume":"72","author":"Capron","year":"1964","journal-title":"La Presse Medicale"},{"key":"S0031182000050459_ref013","first-page":"171","article-title":"In vitro studies of inflammatory bowel diseases, Surface receptors of the mononuclear cell required to lyse allogeneic colonic epithelial cells","volume":"70","author":"Stobb","year":"1976","journal-title":"Gastroenterology"},{"key":"S0031182000050459_ref009","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj1954.23.251"},{"key":"S0031182000050459_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(77)90256-1"},{"key":"S0031182000050459_ref005","doi-asserted-by":"crossref","first-page":"260","DOI":"10.4049\/jimmunol.111.1.260","article-title":"Hypersensitivity to Ascaris antigens. VIII. Characterization of a highly purified allergen","volume":"111","author":"Hussain","year":"1973","journal-title":"Journal of Immunology"},{"key":"S0031182000050459_ref006","first-page":"60","volume-title":"In Inflammatory Bowel Diseases","author":"Kraft","year":"1975"},{"key":"S0031182000050459_ref004","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1964.tb14213.x"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000050459","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T09:18:59Z","timestamp":1710407939000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000050459\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,4]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1983,4]]}},"alternative-id":["S0031182000050459"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000050459","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,4]]}}}