{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T23:27:06Z","timestamp":1698190026186},"reference-count":25,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":8223,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1986,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Excretions and secretions (E\u2013S) were collected from a series of developmental stages of<jats:italic>Litomosoides carinii<\/jats:italic>maintained<jats:italic>in vitro<\/jats:italic>. Measurement of the protein content of E\u2013S obtained from each stage indicates that the rate of production of E\u2013S varies enormously during development of the worm. E\u2013S was iodinated using both Iodogen and the Bolton and Hunter Reagent and was also biosynthetically labelled by incubating worms in the presence of [<jats:sup>35<\/jats:sup>S]methionine and [<jats:sup>3<\/jats:sup>H]leucine. Attempts to biosynthetically label E\u2013S of mature worms and microfilariae with [<jats:sup>3<\/jats:sup>H]glucose were unsuccessful. Examination of radio-isotope labelled E\u2013S by SDS\u2013PAGE revealed that some components were sex specific and that the differences in total E\u2013S production during development were due to the existence of both stage-specific components and components whose rate of release varied during parasite maturation. Antigenic characterization of E\u2013S, carried out by immunoprecipitation in combination with SDS\u2013PAGE, indicated that E\u2013S consists of immunogenic components, a molecule which is probably a non-immunogenic parasite product, and host albumin. The implications of these findings for the construction of diagnostic tests to detect products of human filarial parasites are discussed.<\/jats:p>","DOI":"10.1017\/s0031182000051489","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:18:46Z","timestamp":1244218726000},"page":"317-331","source":"Crossref","is-referenced-by-count":19,"title":["Quantitative and qualitative change in production of excretions\/secretions by<i>Litomosoides carinii<\/i>during development in the jird (<i>Meriones unguiculatus<\/i>)"],"prefix":"10.1017","volume":"93","author":[{"given":"W.","family":"Harnett","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Meghji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"Worms","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. M. 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