{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:55:25Z","timestamp":1761540925648},"reference-count":26,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":8223,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1986,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Onchocerca volvulus<\/jats:italic>proteins labelled with<jats:sup>125<\/jats:sup>I were immunoprecipitated with onchocerciasis and lymphatic filariasis human serum pools in order to differentiate between cross-reacting and non-cross-reacting antigens. Analysis of the immunoprecipitates by two-dimensional gel electrophoresis revealed that all high molecular weight (<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>)<jats:italic>O. volvulus<\/jats:italic>antigens cross-reacted with the lymphatic filariasis serum pools. We observed, however, that at least 8<jats:italic>0. volvulus<\/jats:italic>antigens were specifically immunoprecipitated only by the onchocerciasis serum pools, with M<jats:sub>r<\/jats:sub>ranging from 20000 up to 43000 Daltons. These results suggest that the lower M<jats:sub>r<\/jats:sub><jats:italic>O. volvulus<\/jats:italic>antigens are more species specific than the other antigens. The significance of these findings for the immunodiagnosis of onchocerciasis is discussed.<\/jats:p>","DOI":"10.1017\/s0031182000051556","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:18:46Z","timestamp":1244218726000},"page":"389-399","source":"Crossref","is-referenced-by-count":14,"title":["Identification of non-cross-reacting antigens of<i>Onchocerca volvulus<\/i>with lymphatic filariasis serum pools"],"prefix":"10.1017","volume":"93","author":[{"given":"E.","family":"Lobos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Weiss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000051556_ref024","first-page":"373","article-title":"Detection of IgE binding Onchocerca volvulus antigens after electrophoretic transfer and immuno-enzyme reaction","volume":"39","author":"Weiss","year":"1982","journal-title":"Acta Tropica"},{"key":"S0031182000051556_ref020","first-page":"428","article-title":"Isolation of living adult Onchocerca volvulus from nodules","volume":"28","author":"Schulz-Key","year":"1977","journal-title":"Tropenmedizin und Parasitologie"},{"key":"S0031182000051556_ref019","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1980.29.563"},{"key":"S0031182000051556_ref017","doi-asserted-by":"publisher","DOI":"10.1159\/000233329"},{"key":"S0031182000051556_ref015","first-page":"413","article-title":"Specific cellular immune unresponsiveness in human filariasis","volume":"33","author":"Ottesen","year":"1977","journal-title":"Immunology"},{"key":"S0031182000051556_ref014","doi-asserted-by":"crossref","first-page":"4407","DOI":"10.1016\/S0021-9258(19)41496-8","article-title":"High resolution two dimensional electrophoresis of proteins","volume":"250","author":"O'Farrell","year":"1975","journal-title":"Journal of Biological Chemistry"},{"key":"S0031182000051556_ref011","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"Journal of Biological Chemistry"},{"key":"S0031182000051556_ref008","first-page":"567","article-title":"Excretory and somatic antigens in the diagnosis of human filariasis","volume":"56","author":"Kaushal","year":"1984","journal-title":"Clinical and Experimental Immunology"},{"key":"S0031182000051556_ref007","first-page":"645","volume-title":"Manual of Clinical Microbiology","author":"Kagan","year":"1974"},{"key":"S0031182000051556_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90168-3"},{"key":"S0031182000051556_ref005","doi-asserted-by":"publisher","DOI":"10.1042\/bj0890114"},{"key":"S0031182000051556_ref004","first-page":"67","article-title":"Cross-reaction among filarial species in hemagglutination test","volume":"40","author":"Fujita","year":"1970","journal-title":"Japanese Journal of Experimental Medicine"},{"key":"S0031182000051556_ref002","unstructured":"Forchhammer J. 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