{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T02:19:41Z","timestamp":1744165181309},"reference-count":30,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":9926,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1982,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Two hybridomas secreting monoclonal IgM antibody to<jats:italic>Schistosoma mansoni<\/jats:italic>have been isolated following fusion of spleen cells from Balb\/c mice immunized with living<jats:italic>S. mansoni<\/jats:italic>and NS1 myeloma cells. One monoclonal IgM antibody (WP66.4) mediated about the same level of passive protection against a challenge infection as immune serum from mice with a chronic<jats:italic>S. mansoni<\/jats:italic>infection. The other monoclonal antibody (WP66.2) did not give a significant level of passive protection. This result indicates that the effective monoclonal antibody recognizes an antigen which may be a valuable candidate for experimental vaccination.<jats:italic>In vitro<\/jats:italic>one monoclonal antibody (WP66.4) caused a much higher level of complement-dependent cytotoxicity than the other (WP66.2), suggesting a possible mechanism for the effect observed<jats:italic>in vivo<\/jats:italic>. With indirect immunofluorescence both monoclonal antibodies reacted with surface determinants on living<jats:italic>S. mansoni<\/jats:italic>schistosomula, adult worms and miracidia but these determinants were not detected on cercariae or lung schistosomula. Neither monoclonal antibody cross-reacted with<jats:italic>S. haematobium<\/jats:italic>schistosomula or<jats:italic>Fasciola hepatica<\/jats:italic>metacercariae, indicating a possible use for these reagents in differential diagnosis of<jats:italic>S. mansoni<\/jats:italic>infections.<\/jats:p>","DOI":"10.1017\/s0031182000051684","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:40:22Z","timestamp":1244220022000},"page":"83-91","source":"Crossref","is-referenced-by-count":61,"title":["Passive immunization of mice against<i>Schistosoma mansoni<\/i>with an IgM monoclonal antibody"],"prefix":"10.1017","volume":"84","author":[{"given":"M. A.","family":"Smith","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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