{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T09:13:18Z","timestamp":1770714798966,"version":"3.49.0"},"reference-count":26,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":9684,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1982,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Microfilariae of<jats:italic>Onchocerca lienalis<\/jats:italic>were obtained from the umbilical skin of naturally infected cattle and were injected into mice. Maximum numbers of microfilariae were recovered from the skin and ears of mice when using the subcutaneous route of inoculation. Microfilariae were distributed throughout the pinna of the ear but were concentrated towards the tip where histological sections showed them to be in the dermis and adipose tissue. Using the number of parasites recovered from the ears as an index of the intensity of infection it was found that inbred CBA\/H<jats:italic>T6T6<\/jats:italic>mice were one of the most susceptible of 11 strains of mice examined. No difference in susceptibility was found between male and female CBA mice of the same age, but marked differences were demonstrated between male CBA mice of different ages. After infection with 5000 microfilariae the recovery of parasites from the ears increased rapidly to a peak at day 35 when 10% of the inoculum was recovered, and thereafter declined up to day 242. Over a range of inoculation doses examined it was found that there was a direct, linear relationship between the number of microfilariae recovered from the ears and the number in the inoculated dose. CBA mice showed marked resistance to reinfection with microfilariae. Six days after challenge with a secondary infection recoveries of microfilariae from the ears were only 26% of the level in challenge controls and fell to 3% of the level of controls by day 35. It is concluded that the model of<jats:italic>O. lienalis<\/jats:italic>microfilariae in CBA mice shows considerable promise as a tool for research into immunological responses to skin-dwelling microfilariae, which are the principal cause of pathology in onchocerciasis.<\/jats:p>","DOI":"10.1017\/s0031182000055268","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:36:38Z","timestamp":1244219798000},"page":"283-293","source":"Crossref","is-referenced-by-count":26,"title":["Experimental infection of mice with the microfilariae of<i>Onchocerca lienalis<\/i>"],"prefix":"10.1017","volume":"85","author":[{"given":"S.","family":"Townson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. E.","family":"Bianco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000055268_ref025","unstructured":"Wesley I. V. (1973). Sex hormones and their influence on preferential susceptibility of the male jird (Meriones unguiculatus) to infection by Brugia pahangi (Nematoda: Filaroidea). Ph.D. thesis, University of California, Los Angeles."},{"key":"S0031182000055268_ref020","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00007380"},{"key":"S0031182000055268_ref026","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1964.13.66"},{"key":"S0031182000055268_ref015","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00022677"},{"key":"S0031182000055268_ref008","doi-asserted-by":"publisher","DOI":"10.2307\/3279458"},{"key":"S0031182000055268_ref005","first-page":"109","article-title":"Large scale recovery of Onchocerca microfilariae from naturally infected cattle and horses","volume":"74","author":"Bianco","year":"1980","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"S0031182000055268_ref004","first-page":"82","article-title":"Experimental infection of laboratory rodents and calves with microfilariae of Onchocerca gibsoni","volume":"31","author":"Beveridge","year":"1980","journal-title":"Tropenmedizin und Parasitologie"},{"key":"S0031182000055268_ref003","doi-asserted-by":"publisher","DOI":"10.2307\/3277514"},{"key":"S0031182000055268_ref009","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1978.27.508"},{"key":"S0031182000055268_ref002","doi-asserted-by":"publisher","DOI":"10.2307\/3277796"},{"key":"S0031182000055268_ref001","doi-asserted-by":"publisher","DOI":"10.2307\/3280671"},{"key":"S0031182000055268_ref014","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(66)90164-7"},{"key":"S0031182000055268_ref023","first-page":"219","article-title":"Parasitologische und immunobiologische Untersuchungen \u00fcber die durch Dipetalonema vitae erzeugte Nagetierfilariose","volume":"27","author":"Weiss","year":"1970","journal-title":"Acta tropica"},{"key":"S0031182000055268_ref017","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00022707"},{"key":"S0031182000055268_ref012","first-page":"696","article-title":"A note on the survival of the microfilariae of Mansonella ozzardi","volume":"16","author":"Mazzotti","year":"1957","journal-title":"Bulletin of the World Health Organization"},{"key":"S0031182000055268_ref019","first-page":"101","article-title":"Host hormones and parasite infection","volume":"8","author":"Solomon","year":"1969","journal-title":"International Review of Tropical Medicine"},{"key":"S0031182000055268_ref011","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1949.11685398"},{"key":"S0031182000055268_ref007","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1017\/S0022149X00007136","article-title":"Studies on Onchocerca gutturosa (Neumann, 1910) and its development in Simulium ornatum (Meigen, 1818). 1. Observations on O. gutturosa in cattle in South-east England","volume":"45","author":"Eichler","year":"1971","journal-title":"Journal of Helminthology"},{"key":"S0031182000055268_ref006","first-page":"271","article-title":"Experimental transmission of Onchocerca volvulus from man to a chimpanzee","volume":"56","author":"Duke","year":"1962","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"S0031182000055268_ref024","first-page":"xxxiii","article-title":"Constitution of patency in rodent filariasis (Litomosoides carinii) and its relation to onchocerciasis","volume":"77","author":"Wenk","year":"1978","journal-title":"Parasitology"},{"key":"S0031182000055268_ref010","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000053713"},{"key":"S0031182000055268_ref016","first-page":"25","article-title":"Studies on Brugia pahangi 9. The longevity of microfilariae transfused from cat to cat","volume":"49","author":"Ponnudurai","year":"1975","journal-title":"Journal of Helminthology"},{"key":"S0031182000055268_ref022","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00027814"},{"key":"S0031182000055268_ref021","first-page":"371","article-title":"Studies on Dipetalonema viteae (Filarioidea) 4. Passive transfer of immunity to circulating microfilariae by spleen cells","volume":"30","author":"Tanner","year":"1979","journal-title":"Tropenmedizin und Parasitologic"},{"key":"S0031182000055268_ref018","first-page":"219","article-title":"Observations on the epidemiology of human filariasis in French Oceania","volume":"61","author":"Rosen","year":"1955","journal-title":"American Journal of Hygiene"},{"key":"S0031182000055268_ref013","first-page":"207","article-title":"Studies in filariasis in East Africa. II. Filarial infections in man, animals and mosquitos on the Kenya Coast","volume":"56","author":"Nelson","year":"1962","journal-title":"Transactions of the Royal Society for Tropical Medicine and Hygiene"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000055268","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T07:17:07Z","timestamp":1589872627000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000055268\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,10]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1982,10]]}},"alternative-id":["S0031182000055268"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000055268","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,10]]}}}