{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,10]],"date-time":"2025-02-10T00:10:01Z","timestamp":1739146201877,"version":"3.37.0"},"reference-count":23,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":7980,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1987,6]]},"abstract":"<jats:p>Phosphorylcholine (PC), an immunodominant component of the cell wall of certain bacteria, fungi and nematodes, is known to induce low anti-PC antibody levels during natural infection by<jats:italic>Trichinella spiralis<\/jats:italic>. This article reports a study in which spleen cells from BCF1 mice infected with<jats:italic>Trichinella<\/jats:italic>sp. larvae were found to produce large numbers of direct haemolytic plaques in response to PC conjugated to sheep red blood cells (SRBC) after muscle-encysted larvae had been killed by treatment with mebendazole. Inhibition of the response by PC-chloride, immunodiffusion and immunoelectrophoretic studies with the anti-PC IgA (TEPC-15) and anti-idiotype T15 serum assays showed the plaque-forming cell (PFC) response to be specific for PC. The absence of haemolytic plaques when unconjugated SRBC or TNP-SRBC were used as indicator cells ruled out involvement of a polyclonal response. Greatest anti-PC PFC response was found to be associated with a microsomal fraction designated FCpl, a particulate fraction behaving as a thymus-dependent antigen. The FCpl fractions from all four strains of<jats:italic>Trichinella<\/jats:italic>employed induced anti-PC PFC responses when injected into mice. These results suggest that FCpl is a suitable antigen for use in detailed studies of immune responses to<jats:italic>Trichinella<\/jats:italic>and related parasites.<\/jats:p>","DOI":"10.1017\/s0031182000055888","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:16:22Z","timestamp":1244218582000},"page":"543-553","source":"Crossref","is-referenced-by-count":28,"title":["Immune response to<i>Trichinella<\/i>epitopes: the antiphosphorylcholine plaque-forming cell response during the biological cycle"],"prefix":"10.1017","volume":"94","author":[{"given":"F. M.","family":"Ubeira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Leiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. T.","family":"Santamarina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. G.","family":"Villa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. L.","family":"Sanmart\u00edn-Dur\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000055888_ref022","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.4049\/jimmunol.132.3.1329","article-title":"Heterogeneity of the human phosphocholine-specific B cell repertoire","volume":"132","author":"Stein","year":"1984","journal-title":"Journal of Immunology"},{"key":"S0031182000055888_ref018","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-635505-5.50008-1"},{"key":"S0031182000055888_ref021","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(86)90144-5"},{"key":"S0031182000055888_ref003","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830141115"},{"key":"S0031182000055888_ref011","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(81)90116-8"},{"key":"S0031182000055888_ref009","doi-asserted-by":"publisher","DOI":"10.1126\/science.176.4038.1027"},{"key":"S0031182000055888_ref023","doi-asserted-by":"crossref","first-page":"2429","DOI":"10.4049\/jimmunol.125.6.2429","article-title":"Clonotypes of anti-phosphocholine antibodies induced with Proteus morganii (Potter). 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