{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:29:08Z","timestamp":1762100948506},"reference-count":36,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":8649,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1985,8]]},"abstract":"<jats:p>Antigenic proteins on the surfaces of different developmental stages of<jats:italic>Schistosoma mansoni<\/jats:italic>were radio-iodinated by the lodogen-catalysed method and identified by immunoprecipitation with a panel of antisera. The sera comprised specific immune serum from mice harbouring a chronic schistosome infection or vaccinated with \u03b3-irradiated cercariae; serum from rabbits immunized with adult schistosome tegumental outer membranes or a partially purified M<jats:sub>r<\/jats:sub>32K glycoprotein from adult worm membranes; and a monoclonal antibody recognizing an M<jats:sub>r<\/jats:sub>20 K antigen on the surface of schistosomula. The M<jats:sub>r<\/jats:sub>38\u201332 K glycoproteins were the major antigens identified in surface-labelled cercariae and their probable association with the glycocalyx is discussed. Schistosomula transformed from cercariae either mechanically or by penetration of host skin<jats:italic>in vitro<\/jats:italic>, expressed a similar pattern of surface antigens to that identified for cercariae, but low molecular weight antigens of M<jats:sub>r<\/jats:sub>20, 17 and 15 K were also detected. The M<jats:sub>r<\/jats:sub>38\u201332 K glycoproteins, although present on newly transformed schistoso mula, were progressively replaced with time, by a single dominant glycoprotein (M<jats:sub>r<\/jats:sub>32 K) expressing identical epitopes to those on the Mr 38\u201332 K complex. Moreover, the data confirm that the M<jats:sub>r<\/jats:sub>32 K glycoprotein persists on the tegument after<jats:italic>in vivo<\/jats:italic>maturation and is conserved, together with M<jats:sub>r<\/jats:sub>20 and 15 K antigens, through to the adult stage. New antigens (M<jats:sub>r<\/jats:sub>97 and 25 K) were also detected during<jats:italic>in vivo<\/jats:italic>maturation and were present in late-stage schistosomes recovered from infected hosts. In addition, the enzyme alkaline phosphatase is expressed on the surfaces of 3-week-old liver worms as a dominant antigen (M<jats:sub>r<\/jats:sub>65 K); this feature may be related to nutritional and\/or physiological processes in the tegument of this metabolically active stage of development.<\/jats:p>","DOI":"10.1017\/s0031182000056535","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:23:26Z","timestamp":1244219006000},"page":"83-99","source":"Crossref","is-referenced-by-count":33,"title":["Changes in the surface antigen profile of<i>Schistosoma mansoni<\/i>during maturation from cercaria to adult worm"],"prefix":"10.1017","volume":"91","author":[{"given":"Gilberto","family":"Payares","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane J.","family":"McLaren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. 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