{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:03:27Z","timestamp":1758632607748},"reference-count":19,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":9561,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1983,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Rats and mice were infected with either normal metacercariae or metacercariae \u03b3-irradiated at 3 krad. or 4 krad. Flukes were recovered at various times after infection and their teguments examined using a transmission electron microscope. In normal flukes, the secretory granules TO, Tl and T2 were all seen during tegumental development. The teguments of flukes from mice developed faster than the corresponding teguments in rats. TO granules were present from day 0 to day 10 post-infection (p.i.) in mouse flukes and from day 0 to day 14 p.i. in rat flukes. Tl granules first appeared in mouse flukes by day 4 p.i. but not until day 8 p.i. in rat flukes. T2 granules were seen in mouse flukes 2 days p.i. but not before 6 days p.i. in rat flukes. \u03b3-Irradiation at 4 krad prevented normal tegumental development in flukes from both rats and mice. TO granules were present at all times in flukes from either host. Tl granules were produced in mouse flukes but their appearance was delayed until day 6 p.i. No significant production of T2 granules occurred in flukes from either host. Parasite survival was also affected by \u03b3-irradiation and none of the flukes reached maturity. Flukes from rats died between 10 and 21 days p.i and flukes from mice died between 14 and 28 days p.i. \u03b3-Irradiation of metacercariae at 3 krad. had an extremely variable effect on subsequent tegumental development in both rats and mice. Some flukes developed normally, some showed development associated with \u03b3-irradiation at 4 krad, whilst some showed intermediate development.<\/jats:p>","DOI":"10.1017\/s0031182000057243","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:36:26Z","timestamp":1244219786000},"page":"137-145","source":"Crossref","is-referenced-by-count":9,"title":["<i>Fasciola hepatica<\/i>: development of the tegument of normal and \u03b3-irradiated flukes during infection in rats and mice"],"prefix":"10.1017","volume":"86","author":[{"given":"D. J.","family":"Burden","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. P.","family":"Bland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. L.","family":"Hughes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. C.","family":"Hammet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000057243_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(75)90036-3"},{"key":"S0031182000057243_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(78)90005-5"},{"key":"S0031182000057243_ref011","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/S0034-5288(18)33819-0","article-title":"The recovery after oral infection of immature Fasciola hepatica from the peritoneal cavity of two strains of mice","volume":"15","author":"Harness","year":"1973","journal-title":"Research in Veterinary Science"},{"key":"S0031182000057243_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(80)90012-0"},{"key":"S0031182000057243_ref009","unstructured":"Doy T. G. & Hughes D. L. (1982). Evidence for two distinct mechanisms of resistance in the rat to reinfection with Fasciola hepatica. International Journal for Parasitology (in the Press)."},{"key":"S0031182000057243_ref014","unstructured":"Hughes D. L. (1963). Some studies on the hostr\u2013parasite relations of Fasciola hepatica. Ph.D. thesis, London University."},{"key":"S0031182000057243_ref018","first-page":"4P","article-title":"Fasciola hepatica: the stimulation of excystation","volume":"59","author":"Sewell","year":"1969","journal-title":"Parasitology"},{"key":"S0031182000057243_ref001","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1980.tb00042.x"},{"key":"S0031182000057243_ref008","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1981.tb00387.x"},{"key":"S0031182000057243_ref016","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0034-5288(18)32616-X","article-title":"The different stages of Fasciola hepatica capable of inducing immunity and the susceptibility of various stages to immunological attack in the sensitised rat","volume":"30","author":"Hughes","year":"1981","journal-title":"Research in Veterinary Science"},{"key":"S0031182000057243_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(73)90066-0"},{"key":"S0031182000057243_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(77)90024-8"},{"key":"S0031182000057243_ref007","first-page":"68","article-title":"Preliminary investigations into the killing of newly excysted juveniles of Fasciola hepatica in immune rats","volume":"79","author":"Davies","year":"1979","journal-title":"Parasitology"},{"key":"S0031182000057243_ref017","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/S0034-5288(18)33502-1","article-title":"Resistance in cattle to Fasciola hepatica induced by X-ray attenuated larvae: results from a controlled field trial","volume":"19","author":"Nansen","year":"1975","journal-title":"Research in Veterinary Science"},{"key":"S0031182000057243_ref013","unstructured":"Hughes D. L. (1959). Chemotherapy of experimental Fasciola hepatica infections. M.Sc. thesis, London University."},{"key":"S0031182000057243_ref019","first-page":"71","article-title":"Immunobiologic diagnosis of fascioliasis. II. The in vitro action of immune serum on the young parasitic stage of F. hepatica \u2013 a new precipitin test for fascioliasis","volume":"31","author":"Wikerhauser","year":"1961","journal-title":"Vet. Arh."},{"key":"S0031182000057243_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60435-2"},{"key":"S0031182000057243_ref015","unstructured":"Hughes D. L. , Doy T. G. , Burden D. J. & Oldham G. (1982). Stage-specific immunity in the rat as demonstrated by \u03b3-irradiated Fasciola hepatica. Parasitology (in the Press)."},{"key":"S0031182000057243_ref004","first-page":"23","volume-title":"Isotopes and Radiation in Parasitology","volume":"3","author":"Bitakaramire","year":"1973"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000057243","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,24]],"date-time":"2019-05-24T19:39:27Z","timestamp":1558726767000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000057243\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,2]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1983,2]]}},"alternative-id":["S0031182000057243"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000057243","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,2]]}}}