{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T09:42:56Z","timestamp":1710409376076},"reference-count":49,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":7919,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1987,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Guinea-pigs immunized with equivalent numbers of normal or radiation-attenuated cercariae<jats:italic>of Schistosomamansoni<\/jats:italic>are known todevelop close to complete resistance to reinfection at weeks 12 and 4\u00b75 respectively. We here analyse and compare the immune responses induced by the two populations of cercariae. Both radiation-attenuated and normal parasites of<jats:italic>S. mansoni<\/jats:italic>elicited an extensive germinal centre response in guinea-pigs by week 4\u00b75 post-immunization. The anti-parasite antibody titre and cytotoxic activity of serum from 4\u00b75-week-vaccinated, or 4\u00b75-week-infected guinea-pigs were approximately equal, but sera from 12-week-infected individuals had high titres of anti-parasite antibody, which promoted significant larvicidal activity<jats:italic>in vitro.<\/jats:italic>In all cases, larvicidal activity was mediated by the IgG2 fraction of the immune serum. Lymphocyte transformation tests conducted on splenic lymphocytes from 4\u20135-week vaccinated guinea-pigs revealed maximal stimulation against cercarial, 2-week and 3-week worm antigens, whereas spleen cells from 4\u20135-week-infected guinea-pigs were maximally stimulated by cercarial and 6-week worm antigens. The splenic lymphocyte responses of 12-week infected animals were dramatic against antigens prepared from all life-stages of the parasite.<\/jats:p>","DOI":"10.1017\/s003118200005753x","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:14:53Z","timestamp":1244218493000},"page":"43-59","source":"Crossref","is-referenced-by-count":1,"title":["Analysis and comparison of immune reactivity in guinea-pigs immunized with equivalent numbers of normal or radiation-attenuated cercariae of<i>Schistosoma mansoni<\/i>"],"prefix":"10.1017","volume":"95","author":[{"given":"M. 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