{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T19:21:02Z","timestamp":1757704862878},"reference-count":31,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":7675,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1988,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Praziquantel (500 mg\/kg) administered orally to BALB\/c mice with secondary equine <jats:italic>E. granulosus<\/jats:italic> daily for 21, 30 or 30 + 30 days without the drug resulted in the majority of cysts, using bench criteria of turgidity and eosin exclusion, being assessed as \u2018alive\u2019. Ultrastructural examination of 54 of these \u2018alive\u2019 cysts did not support this conclusion. They all showed increased vesiculation of the germinal layer leading, in many, to the loss of its integrity. Increased mitochondrial numbers occurred frequently. The longer drug treatments appeared to have greater effects on the germinal layer of \u2018alive\u2019 cysts and there was no detectable re-establishment of structural organization within 30 days after drug withdrawal. Subjectively, there was no substantial difference between cysts from 4-month and 9-month infections or between affected peritoneal and hepatic cysts. Tissue from collapsed cysts was necrotic. Peak serum levels of praziquantel (6430\u20136136 \u03bcg\/l) occurred 5\u201310 min after drug administration (500 mg\/kg) and dropped rapidly to less than 10 \u03bcg\/l at 3 h. In an <jats:italic>in vitro<\/jats:italic> study at praziquantel concentrations of 1000 and 5000 \u03bcg\/l over a 10-day period, most cysts were judged \u2018alive\u2019 by bench criteria but showed ultrastructurally a time- and concentration-dependent loss of integrity identical to that seen <jats:italic>in vivo<\/jats:italic>. Turgidity and eosin exclusion therefore underestimate the effect of praziquantel and the results indicate that <jats:italic>in vitro<\/jats:italic> experiments can fulfil a legitimate preliminary role in a hydatid chemotherapy programme.<\/jats:p>","DOI":"10.1017\/s0031182000058327","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:10:59Z","timestamp":1244218259000},"page":"323-336","source":"Crossref","is-referenced-by-count":27,"title":["<i>Echinococcus granulosus<\/i>: the effects of praziquantel, <i>in vivo<\/i> and <i>in vitro<\/i>, on the ultrastructure of equine strain murine cysts"],"prefix":"10.1017","volume":"96","author":[{"given":"K. Sylvia","family":"Richards","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. L.","family":"Morris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Daniels","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. M.","family":"Riley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000058327_ref022","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1985.11811897"},{"key":"S0031182000058327_ref019","doi-asserted-by":"publisher","DOI":"10.1007\/BF03189418"},{"key":"S0031182000058327_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(87)90173-8"},{"key":"S0031182000058327_ref015","first-page":"544","article-title":"In vivo and in vitro experiments on the effects of praziquantel on Schistosoma mansoni","volume":"31","author":"Mehlhorn","year":"1981","journal-title":"Arzneimittel-Forschung\/Drug Research"},{"key":"S0031182000058327_ref008","first-page":"3","article-title":"Effects of praziquantel against eggs of Taenia hydatigena and protoscolices and metacestodes of Echinococcus granulosus","volume":"1","author":"Gemmell","year":"1983","journal-title":"Veterinary Medical Review"},{"key":"S0031182000058327_ref005","doi-asserted-by":"publisher","DOI":"10.1007\/BF00927373"},{"key":"S0031182000058327_ref002","doi-asserted-by":"publisher","DOI":"10.1002\/med.2610030204"},{"key":"S0031182000058327_ref009","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000049738"},{"key":"S0031182000058327_ref004","doi-asserted-by":"publisher","DOI":"10.1007\/BF00927527"},{"key":"S0031182000058327_ref013","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00034441"},{"key":"S0031182000058327_ref023","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000064143"},{"key":"S0031182000058327_ref007","first-page":"1137","article-title":"Results of toxicological studies on praziquantel","volume":"34","author":"Frohberg","year":"1984","journal-title":"Arzneimittel-Forschung \/ Drug Research"},{"key":"S0031182000058327_ref003","doi-asserted-by":"publisher","DOI":"10.1007\/BF00925459"},{"key":"S0031182000058327_ref026","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-282X1985000300003"},{"key":"S0031182000058327_ref024","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000051519"},{"key":"S0031182000058327_ref018","first-page":"8","volume-title":"Penetration of praziquantel into cerebrospinal fluid and cysticerci in human cysticercosis","author":"Overbosch","year":"1984"},{"key":"S0031182000058327_ref001","first-page":"154","article-title":"Pharmacokinetic studies with Droncit in animals using a biological assay","volume":"2","author":"Andrews","year":"1976","journal-title":"Veterinary Medical Reviews"},{"key":"S0031182000058327_ref020","doi-asserted-by":"publisher","DOI":"10.1007\/BF03189426"},{"key":"S0031182000058327_ref016","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/18.6.687"},{"key":"S0031182000058327_ref021","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000001116"},{"key":"S0031182000058327_ref006","first-page":"383","article-title":"Multicentre clinical trials of the benzimidazole carbamates in human echinococcosis","volume":"64","author":"Davis","year":"1986","journal-title":"Bulletin of the World Health Organization"},{"key":"S0031182000058327_ref014","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1982.11687596"},{"key":"S0031182000058327_ref010","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.1978.34477"},{"key":"S0031182000058327_ref025","first-page":"95","article-title":"Alterations of the surface tegument of Opisthorchis viverrini exposed to praziquantel in vitro and in vivo","volume":"15","author":"Sirisinha","year":"1984","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0031182000058327_ref028","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00008488"},{"key":"S0031182000058327_ref030","doi-asserted-by":"publisher","DOI":"10.2307\/3281955"},{"key":"S0031182000058327_ref031","unstructured":"Yao Ping L. & Jun L. (1985). Praziquantel in treatment and investigation with abdominal hydatidosis. Preliminary report of animal experiments and evaluation of 101 clinical cases. XIII Congreso Internacional de Hidatidologia, Madrid, Abstract 286."},{"key":"S0031182000058327_ref012","first-page":"41","article-title":"In vitro effects of Praziquantel on Fibricola seoulensis","volume":"26","author":"Lee","year":"1985","journal-title":"Seoul Journal of Medicine"},{"key":"S0031182000058327_ref027","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1982.31.803"},{"key":"S0031182000058327_ref011","unstructured":"Langston V. C. , Galey F. , Lovell R. & Buck W. B. (1985). Toxicity and therapeutics of monensin: a review. Veterinary Medicine 09 75\u201384."},{"key":"S0031182000058327_ref029","first-page":"605","volume-title":"Biochemistry of Parasites and Host-Parasite Relationships","author":"Verheyen","year":"1976"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000058327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T20:03:46Z","timestamp":1558382626000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000058327\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,4]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1988,4]]}},"alternative-id":["S0031182000058327"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000058327","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,4]]}}}