{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T09:30:46Z","timestamp":1747906246204},"reference-count":14,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":7066,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1989,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>A<jats:italic>Babesia divergens<\/jats:italic>merozoite antigen was purified by affinity chromatography using a monoclonal antibody. Silver staining of SDS-PAGE gels revealed 2 bands of M<jats:sub>r<\/jats:sub>50\u201360 kDa and M<jats:sub>r<\/jats:sub>24\u201329. It is proposed that the M<jats:sub>r<\/jats:sub>24\u201329 kDa band represents the native protein and that it is complexed, perhaps as a dimer or perhaps to host protein to give the M<jats:sub>r<\/jats:sub>50\u201360 kDa band. Peptide analysis of the antigen followed by Western blotting with the monoclonal antibody revealed only an M<jats:sub>r<\/jats:sub>20\u201324 kDa band. After affinity purification the antigen was precipitated with acetone and inoculated subcutaneously, together with complete Freund's adjuvant, into gerbils. Two inoculations were given 3 weeks apart, after which the immunized animals and a group of control animals were challenged with 5 \u00d7 parasitized red cells from a virulent microaerophilus stationary phase (MASP) culture of an homologous strain of<jats:italic>B. divergens<\/jats:italic>. Parasitaemias rose rapidly in control animals and all were dead by the fifth day. Two of the vaccinated animals survived the challenge. Parasitaemias in the remaining two rose slowly but both animals succumbed on the seventh day. Western blotting of merozoites and of the purified antigen, using sera from the surviving animals showed strong reactivity with the M<jats:sub>r<\/jats:sub>24\u201329 kDa band.<\/jats:p>","DOI":"10.1017\/s0031182000059059","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:45:46Z","timestamp":1244213146000},"page":"341-348","source":"Crossref","is-referenced-by-count":5,"title":["A monoclonal antibody-derived antigen of<i>Babesia divergens<\/i>: characterization and investigation of its ability to protect gerbils against virulent homologous challenge"],"prefix":"10.1017","volume":"99","author":[{"given":"C. M.","family":"Winger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. U.","family":"Canning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. D.","family":"Culverhouse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000059059_ref013","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1128\/IAI.48.1.109-113.1985","article-title":"Protective vaccination against virulent Babesia bovis with a low-molecular-weight antigen","volume":"48","author":"Wright","year":"1985","journal-title":"Infection and Immunity"},{"key":"S0031182000059059_ref011","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(87)90075-6"},{"key":"S0031182000059059_ref010","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000053415"},{"key":"S0031182000059059_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(84)90041-4"},{"key":"S0031182000059059_ref006","first-page":"264","article-title":"Exoantigens of Babesia","author":"Ristic","year":"1988","journal-title":"Babesiosis of Domestic Animals and Man"},{"key":"S0031182000059059_ref005","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.4049\/jimmunol.138.7.2298","article-title":"Antibodies define multiple proteins with epitopes exposed on the surface of live Babesia bigemina merozoites","volume":"138","author":"Mcelwain","year":"1987","journal-title":"Journal of Immunology"},{"key":"S0031182000059059_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(86)90150-0"},{"key":"S0031182000059059_ref004","doi-asserted-by":"publisher","DOI":"10.1126\/science.7355284"},{"key":"S0031182000059059_ref002","doi-asserted-by":"publisher","DOI":"10.1038\/256495a0"},{"key":"S0031182000059059_ref003","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"S0031182000059059_ref009","first-page":"3418","article-title":"Enzyme immunoassays in diagnostic medicine. Theory and practice","volume":"53","author":"Voller","year":"1976","journal-title":"Bulletin of the World Health Organization"},{"key":"S0031182000059059_ref012","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/S0034-5288(18)31128-7","article-title":"A strain of Babesia divergens attenuated after long term culture","volume":"46","author":"Winger","year":"1989","journal-title":"Research in Veterinary Science"},{"key":"S0031182000059059_ref014","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1128\/IAI.41.1.244-250.1983","article-title":"Babesia bovis: isolation of a protective antigen by using monoclonal antibodies","volume":"41","author":"Wright","year":"1983","journal-title":"Infection and Immunity"},{"key":"S0031182000059059_ref001","first-page":"192","volume-title":"Methods in Molecular Biology","volume":"1","author":"Gooderham","year":"1984"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000059059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T14:32:15Z","timestamp":1685025135000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000059059\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,12]]},"references-count":14,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1989,12]]}},"alternative-id":["S0031182000059059"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000059059","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,12]]}}}