{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:21:15Z","timestamp":1791354075022,"version":"4.3.1"},"reference-count":16,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":6397,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1991,10]]},"abstract":"<jats:p>Three isolates of<jats:italic>Babesia divergens<\/jats:italic>have been cultured continuously for 6 months in rat erythrocytes using the candle jar technique (Trager &amp; Jensen, 1976). One isolate was already rat-adapted, the other two became adapted to rats through continuous culture in rat erythrocytes. Parasites were cultured in rat erythrocytes in RPMI medium supplemented with 20% foetal calf serum. The highest parasitaemia obtained was 35% and multiparasitization of red blood cells was often observed. Cultures of<jats:italic>B. divergens<\/jats:italic>remained infective to splenectomized rats. Cultures with high parasitaemias contained a large number of extracellular merozoites. When separated from the red blood cells, these extracellular merozoites retained their infectivity.<\/jats:p>","DOI":"10.1017\/s0031182000059436","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:29:25Z","timestamp":1244212165000},"page":"165-170","source":"Crossref","is-referenced-by-count":16,"title":["The adaptation of three isolates of<i>Babesia divergens<\/i>to continuous culture in rat erythrocytes"],"prefix":"10.1017","volume":"103","author":[{"given":"N.","family":"Ben Musa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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