{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:49:09Z","timestamp":1762271349938},"reference-count":11,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":6701,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1990,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Mass-scale production of<jats:italic>Plasmodium vivax<\/jats:italic>sporozoites in<jats:italic>Anopheles stephensi<\/jats:italic>was achieved using the chimpanzee (<jats:italic>Pan troglodytes<\/jats:italic>) as a source of infective blood. Membrane feeding was as successful as feeding mosquitoes directly on the animal so long as the time between drawing the blood and feeding was restricted to 45 min. Longer delays such as 2\u20133 h resulted in loss of infectivity in terms of oocyst production. The selected strain of<jats:italic>A. stephensi<\/jats:italic>was highly susceptible to<jats:italic>P. vivax<\/jats:italic>(Chesson strain). A strain of<jats:italic>A. stephensi<\/jats:italic>relatively refractory to<jats:italic>P. falciparum<\/jats:italic>showed no cross-refractoriness to<jats:italic>P. vivax<\/jats:italic>. Mixed infections of<jats:italic>P. falciparum<\/jats:italic>and<jats:italic>P. vivax<\/jats:italic>did not interfere with each other in their development in<jats:italic>A. stephensi<\/jats:italic>. A second normal blood meal to mosquitoes infected with<jats:italic>P. vivax<\/jats:italic>increased the yield of salivary gland sporozoites.<\/jats:p>","DOI":"10.1017\/s0031182000060492","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:36:07Z","timestamp":1244212567000},"page":"317-320","source":"Crossref","is-referenced-by-count":11,"title":["Large-scale production of<i>Plasmodium vivax<\/i>sporozoites"],"prefix":"10.1017","volume":"101","author":[{"given":"T.","family":"Ponnudurai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. H. W.","family":"Lensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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P. & Gwadz R. W. (1980). The transmission by mosquitoes of Plasmodia in the laboratory.. kyyIn Malaria. vol2, (ed. Kreier, J. P.), pp. 153\u2013234. London:Academic Press.","DOI":"10.1016\/B978-0-12-426102-0.50010-5"},{"key":"S0031182000060492_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(82)90116-X"},{"key":"S0031182000060492_ref002","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1986.35.56"},{"key":"S0031182000060492_ref001","first-page":"645","article-title":"The employment of multiple infected Anopheles quadrimaculatus to effect inoculation with Plasmodium vivax and Plasmodium falciparum","volume":"21","author":"Boyd","year":"1973","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"S0031182000060492_ref007","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000062065"},{"key":"S0031182000060492_ref003","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.1989.tb00473.x"},{"key":"S0031182000060492_ref005","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1128\/IAI.55.2.369-372.1987","article-title":"Malaria transmission blocking immunity induced by natural infections of Plasmodium vivax in humans","volume":"55","author":"Mendis","year":"1987","journal-title":"Infection and Immunity"},{"key":"S0031182000060492_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(89)90119-7"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000060492","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T06:47:33Z","timestamp":1589870853000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000060492\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,12]]},"references-count":11,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,12]]}},"alternative-id":["S0031182000060492"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000060492","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,12]]}}}