{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T01:02:12Z","timestamp":1698714132867},"reference-count":15,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":7249,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1989,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:sup>125<\/jats:sup>I-<jats:italic>Schistosoma mansoni<\/jats:italic>schistosomulum surface antigens were immunoprecipitated with human antibodies from individual Egyptian patients diagnosed as being either acutely or chronically infected with<jats:italic>S. mansoni<\/jats:italic>. Both sets of patients were found to have IgG antibodies in their sera capable of immunoprecipitating the major<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>&gt; 200, 38 and 32K antigens. However, the immunoprecipitation of the<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>200K antigen was found to constitute a significantly greater proportion of the total precipitate achieved with acute sera than with chronic sera. The<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>38 and 32K antigens were more variably precipitated by the acute sera than the chronic sera but the proportion of the total precipitation that these two antigens constituted was not found to be significantly different between the two sets of sera. Immunoprecipitation with pooled antibodies absorbed with egg and adult worm homogenates which had been treated to remove either carbohydrate or polypeptide epitopes demonstrated that the<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>&gt; 200K antigen was the principal target of egg-cross-reactive anti-carbohydrate antibody amongst the antigens detected. The<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>38 and 32K antigens were found to be precipitated by antibodies to protease-sensitive and periodate-insensitive polypeptide epitopes. These results are consistent with egg-cross-reactive anti-carbohydrate IgG antibody making a greater contribution to schistosomulum surface recognition in acute infection than in chronic infection. Indeed the presence of a higher level of egg-cross-reactive and anti-carbohydrate antibody directed against schistosomulum surface epitopes in an acute serum pool than in a chronic serum pool was confirmed by measurement of antibody binding to whole schistosomula.<\/jats:p>","DOI":"10.1017\/s0031182000061503","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:52:29Z","timestamp":1244213549000},"page":"417-424","source":"Crossref","is-referenced-by-count":23,"title":["Antibody to carbohydrate and polypeptide epitopes on the surface of schistosomula of<i>Schistosoma mansoni<\/i>in Egyptian patients with acute and chronic schistosomiasis"],"prefix":"10.1017","volume":"98","author":[{"given":"P.","family":"Omer Ali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Mansour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. N.","family":"Woody","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. R.","family":"Smithers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J. G.","family":"Simpson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000061503_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(87)90091-3"},{"key":"S0031182000061503_ref007","first-page":"265","volume-title":"The Biology of Schistosomes","author":"Kelly","year":"1987"},{"key":"S0031182000061503_ref012","doi-asserted-by":"crossref","first-page":"3273","DOI":"10.4049\/jimmunol.140.9.3273","article-title":"Analysis of the anti-Schistosoma mansoni surface antibody response during murine infection and its potential contribution to protective immunity","volume":"140","author":"Omer Ali","year":"1988","journal-title":"Journal of Immunology"},{"key":"S0031182000061503_ref010","article-title":"Variable species and stage specificities of schistosomulum surface epitopes recognized by mice vaccinated with highly irradiated cercariae","author":"Omer Ali","year":"1989","journal-title":"Parasite Immunology"},{"key":"S0031182000061503_ref009","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1988.38.540"},{"key":"S0031182000061503_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(85)90391-8"},{"key":"S0031182000061503_ref003","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/149.2.227"},{"key":"S0031182000061503_ref011","doi-asserted-by":"crossref","first-page":"3601","DOI":"10.4049\/jimmunol.137.11.3601","article-title":"A major role for carbohydrates preferentially recognised by chronically infected mice in the determination of Schistosoma mansoni schistosomulum surface antigenicity","volume":"137","author":"Omer Ali","year":"1986","journal-title":"Journal of Immunology"},{"key":"S0031182000061503_ref008","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1002\/j.1460-2075.1984.tb01786.x","article-title":"Cell-free synthesis of Schistosoma mansoni surface antigens: stage specificity of their expression","volume":"3","author":"Knight","year":"1984","journal-title":"EMBO Journal"},{"key":"S0031182000061503_ref014","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(86)90032-5"},{"key":"S0031182000061503_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(78)91322-0"},{"key":"S0031182000061503_ref015","first-page":"185","volume-title":"The Biology of Schistosomes","author":"Von Lichtenberg","year":"1987"},{"key":"S0031182000061503_ref004","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830180119"},{"key":"S0031182000061503_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(74)90076-9"},{"key":"S0031182000061503_ref006","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.4049\/jimmunol.135.3.2121","article-title":"Immune responses during human schistosomiasis mansoni. xi. Immunologic status of patients with acute infections and after treatment","volume":"135","author":"Gazzinelli","year":"1985","journal-title":"Journal of Immunology"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000061503","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T14:32:49Z","timestamp":1685025169000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000061503\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,6]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1989,6]]}},"alternative-id":["S0031182000061503"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000061503","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,6]]}}}