{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T23:48:08Z","timestamp":1787960888363,"version":"build-2784847793"},"reference-count":52,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":6214,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1992,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Two sets of oligonucleotide primers, one derived from a repetitive sequence and the other from the gene encoding a 67 kDa sporozoite antigen of <jats:italic>Theileria parva<\/jats:italic>, were used to amplify parasite DNA from the blood of <jats:italic>T. parva<\/jats:italic>-infected carrier cattle using the polymerase chain reaction (PCR). PCR amplification products were obtained from 15 carrier cattle infected with one of 4 different <jats:italic>T. parva<\/jats:italic> stocks. Successful amplifications were performed using DNA from 2 cattle infected with <jats:italic>T. p. parva<\/jats:italic> Pemba Mnarani, 10 cattle infected with <jats:italic>T. p. parva<\/jats:italic> Marikebuni, 2 cattle infected with <jats:italic>T. p. bovis<\/jats:italic> Boleni and 1 animal infected with <jats:italic>T. p. lawrencei<\/jats:italic> 7014. No amplification products were obtained from any of 7 cattle which had been infected with the <jats:italic>T. p. parva<\/jats:italic> Muguga stock. A synthetic oligonucleotide, which hybridized specifically to <jats:italic>T. p. parva<\/jats:italic> Marikebuni DNA among 6 <jats:italic>T. parva<\/jats:italic> stocks tested, was designed using sequence data from within the region of the <jats:italic>T. parva<\/jats:italic> genome amplified by the repetitive sequence primers. The oligonucleotide was used to probe PCR products and to increase the sensitivity and specificity of carrier animal detection. Southern blot analysis using a <jats:italic>T. parva<\/jats:italic> repetitive sequence probe demonstrated the existence of restriction fragment length polymorphisms between parasites isolated from <jats:italic>T. p. parva<\/jats:italic> Marikebuni-infected carrier cattle. The use of the PCR and other methods of carrier animal detection are discussed.<\/jats:p>","DOI":"10.1017\/s0031182000061655","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:25:25Z","timestamp":1244211925000},"page":"215-232","source":"Crossref","is-referenced-by-count":77,"title":["Detection of a carrier state in <i>Theileria parva<\/i>-infected cattle by the polymerase chain reaction"],"prefix":"10.1017","volume":"104","author":[{"given":"R.","family":"Bishop","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Sohanpal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. P.","family":"Kariuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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