{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T17:42:57Z","timestamp":1758476577271},"reference-count":17,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":6214,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1992,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>An antibody competition enzyme-linked immunosorbent assay (ELISA) using 4 different monoclonal antibodies (MAb) raised against major antigens (Antigen 5 and Antigen B) of <jats:italic>Echinococcus granulosus<\/jats:italic> was evaluated for the diagnosis of human hydatidosis. The competition assay, using anti-Ag5 MAb 24.14, detected specific antibodies in 70% (131\/188) of sera from patients with surgically confirmed <jats:italic>E. granulosus<\/jats:italic> infection and 38\u00b75% (10\/26) of sera from patients with <jats:italic>E. multilocularis<\/jats:italic> infection. None of the sera from patients with <jats:italic>Taenia solium<\/jats:italic> cysticercosis (10), <jats:italic>T. saginata<\/jats:italic> (2), filariasis (22), strongyloidiasis (19), fascioliasis (4), bilharziasis (4) and amoebiasis (2) tested were positive using a cut-off point established through reaction between MAb 24.14 and normal human sera. The combined use of the MAb 24.14-based competition ELISA with the conventional antibody-binding assay provides a highly sensitive (92\u00b78%) and specific screening system for human hydatid disease diagnosis.<\/jats:p>","DOI":"10.1017\/s0031182000061813","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T10:25:25Z","timestamp":1244197525000},"page":"357-361","source":"Crossref","is-referenced-by-count":19,"title":["Evaluation of a monoclonal antibody-based competition ELISA for the diagnosis of human hydatidosis"],"prefix":"10.1017","volume":"104","author":[{"given":"D.","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. W.","family":"Lightowlers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. D.","family":"Rickard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000061813_ref016","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1975.24.298"},{"key":"S0031182000061813_ref015","doi-asserted-by":"publisher","DOI":"10.1007\/BF00380463"},{"key":"S0031182000061813_ref013","first-page":"459","article-title":"Applications of the enzyme-linked immunosorbent assay (ELISA) for the diagnosis of filariasis and echinococcosis","volume":"31","author":"Speiser","year":"1980","journal-title":"Tropenmedizin und Parasitologie"},{"key":"S0031182000061813_ref012","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1980.29.609"},{"key":"S0031182000061813_ref011","doi-asserted-by":"publisher","DOI":"10.3109\/00313028409059107"},{"key":"S0031182000061813_ref010","doi-asserted-by":"publisher","DOI":"10.3109\/00313028409059106"},{"key":"S0031182000061813_ref009","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1983.32.114"},{"key":"S0031182000061813_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(89)90149-7"},{"key":"S0031182000061813_ref006","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1987.37.609"},{"key":"S0031182000061813_ref003","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000085310"},{"key":"S0031182000061813_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(87)90418-4"},{"key":"S0031182000061813_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(86)90025-3"},{"key":"S0031182000061813_ref008","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1984.tb07200.x"},{"key":"S0031182000061813_ref017","doi-asserted-by":"publisher","DOI":"10.2307\/3280010"},{"key":"S0031182000061813_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(82)90140-5"},{"key":"S0031182000061813_ref014","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1978.27.554"},{"key":"S0031182000061813_ref001","first-page":"27","volume-title":"Journ\u00e9e Lyonnaises d'Hydatidologie","author":"Capron","year":"1967"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000061813","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,16]],"date-time":"2019-05-16T18:03:14Z","timestamp":1558029794000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000061813\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,4]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,4]]}},"alternative-id":["S0031182000061813"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000061813","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,4]]}}}