{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T07:19:20Z","timestamp":1648538360043},"reference-count":18,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":8406,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1986,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>A variety of morphological forms developed during monophasic culture of ruptured brood capsules of equine <jats:italic>Echinococcus granulosus<\/jats:italic>. Attached to some protoscoleces were small posterior bladders, with a surface ultrastructure of truncated microtriches, and these never developed a laminated layer. In contrast, small free vesicles of similar size and ultrastructure, but devoid of an attached protoscolex, produced a layer by 20\u201330 days. The surface ultrastructure of both these forms is similar to that of the protoscolex attachment stalk and the brood capsule lining, and posterior bladders and small free vesicles are considered to be derived from portions of the stalk\u00b1part of the brood capsule lining retained at protoscolex release. Also present were large vesicles interpreted from their size and surface anatomy as ruptured, everted brood capsules. When devoid of attached protosoleces these formed cysts each with a laminated layer, whereas similar vesicles with several attached protoscoleces did not develop a layer, or only very occasionally after 70 or more days. In contrast, a few large vesicles with a single attached protoscolex produced a laminated layer. The possibility that protoscolex attachment to stalk remnants or brood capsules inhibits the cystic development of these structures is discussed.<\/jats:p>","DOI":"10.1017\/s0031182000064143","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:20:47Z","timestamp":1244218847000},"page":"379-390","source":"Crossref","is-referenced-by-count":24,"title":["<i>In vitro<\/i> development of hydatid cysts from posterior bladders and ruptured brood capsules of equine <i>Echinococcus granulosus<\/i>"],"prefix":"10.1017","volume":"92","author":[{"given":"M. T.","family":"Rogan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. Sylvia","family":"Richards","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000064143_ref001","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/1968435561"},{"key":"S0031182000064143_ref011","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000049064"},{"key":"S0031182000064143_ref015","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000027256"},{"key":"S0031182000064143_ref010","first-page":"99","article-title":"Estudio de Ia capa laminada, membranas germinativas y tegumento del protoescolex del Equinococcus granulosus en su localizacion humana","volume":"2","author":"Lopez-Campos","year":"1978","journal-title":"Morfologia normal y patologia"},{"key":"S0031182000064143_ref018","first-page":"85","article-title":"Studies on Echinococcus. XIII. Observations on the vesicular development of the scolex of Echinococcus multilocularis in vitro","volume":"10","author":"Yamashita","year":"1962","journal-title":"Japanese Journal of Veterinary Research"},{"key":"S0031182000064143_ref005","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00004582"},{"key":"S0031182000064143_ref003","doi-asserted-by":"publisher","DOI":"10.2307\/3281368"},{"key":"S0031182000064143_ref014","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000050599"},{"key":"S0031182000064143_ref007","first-page":"372","article-title":"Vitalidid y evolutividad de los escolices hidaticos in vivo e in vitro","volume":"48","author":"Gurri","year":"1963","journal-title":"Anales-Facultad de Medcina de Montevideo"},{"key":"S0031182000064143_ref013","first-page":"191","article-title":"Contributo all\u00b4 studio delle colture in vitro degli scolici de Echinococcus granulosus","volume":"7","author":"Pauluzzi","year":"1965","journal-title":"Annali Slavo"},{"key":"S0031182000064143_ref002","first-page":"292","article-title":"Cystic development of protoscoleces and brood capsules of Echinococcus granulosus in cell cultures and cell free media","volume":"40","author":"Brudnjak","year":"1970","journal-title":"Veterinarski Archiv, Zagreb"},{"key":"S0031182000064143_ref004","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/1927051001"},{"key":"S0031182000064143_ref006","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00021532"},{"key":"S0031182000064143_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(76)90083-7"},{"key":"S0031182000064143_ref009","doi-asserted-by":"publisher","DOI":"10.2307\/3279219"},{"key":"S0031182000064143_ref012","doi-asserted-by":"publisher","DOI":"10.2307\/3276582"},{"key":"S0031182000064143_ref016","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000071936"},{"key":"S0031182000064143_ref017","first-page":"137","article-title":"Echinococcus granulosus and E","volume":"20","author":"Smyth","year":"1979","journal-title":"Angewandte Parasitologie"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000064143","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,22]],"date-time":"2019-05-22T06:40:26Z","timestamp":1558507226000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000064143\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,4]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1986,4]]}},"alternative-id":["S0031182000064143"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000064143","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,4]]}}}