{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T15:10:34Z","timestamp":1685027434789},"reference-count":42,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":6519,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1991,6]]},"abstract":"<jats:p>Antigens were extracted from the epicuticle\/cuticle of intact female<jats:italic>Onchocerca volvulus<\/jats:italic>using 2% 2-\u03b2-mercaptoethanol and 1% SDS. In Western blot analysis a human infection serum selected for its high antibody titre against whole worm homogenates did not recognize any component solubilized by 1% SDS. However, the same serum did bind at least 7 antigens among the material extracted with 2-\u03b2-mercaptoethanol. These antigens have apparent molecular weights (<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>) of: 15 000, 18 000, 28 000, 78 000, 98 000, 120 000 and 200 000. In ELISA using this preparation as target antigen, 151 out of 153 human infection sera gave positive results. An<jats:italic>Onchocerca<\/jats:italic>-specific IgG1 monoclonal antibody, designated Cam1, recognized the 28 000<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>antigen, which is the most prominent antigen detected by Western blot analysis using human infection sera. In ELISA, using material affinity-purified with Cam1 as target antigen, 149 out of 153 human infection sera gave a positive IgG response. From a cDNA library three expressing clones were isolated with a rabbit serum raised against 2-\u03b2-mercaptoethanol solubilized material. One of these clones was recognized by the monoclonal antibody Cam1.<\/jats:p>","DOI":"10.1017\/s0031182000064416","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:32:56Z","timestamp":1244212376000},"page":"437-444","source":"Crossref","is-referenced-by-count":2,"title":["Partial characterization of 2-\u03b2-mercaptoethanol-soluble surface-associated antigens of<i>Onchocerca volvulus<\/i>"],"prefix":"10.1017","volume":"102","author":[{"given":"F.","family":"Engelbrecht","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Braun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Connor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Downham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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