{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T09:59:26Z","timestamp":1792231166932,"version":"4.4.0"},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":4997,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1995,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>A haemoprotein released <jats:italic>in vitro<\/jats:italic> by adult <jats:italic>Fasciola hepatica<\/jats:italic> was purified by gel filtration chromatography on Sephacryl S-200 and ion-exchange chromatography on DEAE-Sepharose. The molecule, with an apparent molecular weight of &gt; 200 kDa, contains a haem group and has absorption spectra characteristics similar to haemoglobins. N-terminal amino acid sequence analysis revealed no similarity between the <jats:italic>F. hepatica<\/jats:italic> haemoglobin and other vertebrate or invertebrate haemoglobins. Antibodies to the haemoglobin molecule can be detected in the sera of <jats:italic>F. hepatica<\/jats:italic>-infected bovines as early as 1 week after infection.<\/jats:p>","DOI":"10.1017\/s0031182000064969","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T09:40:59Z","timestamp":1244194859000},"page":"209-215","source":"Crossref","is-referenced-by-count":37,"title":["Isolation of <i>Fasciola hepatica<\/i> haemoglobin"],"prefix":"10.1017","volume":"111","author":[{"given":"S.","family":"McGonigle","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. P.","family":"Dalton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000064969_ref020","first-page":"1","article-title":"The structure of invertebrate extracellular hemoglobin (erythrocruorins and chlorocruorins)","volume":"82","author":"Vinogradov","year":"1985","journal-title":"Comparative Biochemical Physiology"},{"key":"S0031182000064969_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(84)90121-X"},{"key":"S0031182000064969_ref013","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000053786"},{"key":"S0031182000064969_ref006","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.tb18969.x"},{"key":"S0031182000064969_ref019","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1978.tb12460.x"},{"key":"S0031182000064969_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(93)90082-Q"},{"key":"S0031182000064969_ref005","doi-asserted-by":"publisher","DOI":"10.2307\/3277394"},{"key":"S0031182000064969_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(63)90077-6"},{"key":"S0031182000064969_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(75)90036-3"},{"key":"S0031182000064969_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(81)90052-9"},{"key":"S0031182000064969_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(80)90033-8"},{"key":"S0031182000064969_ref001","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-349-86119-4"},{"key":"S0031182000064969_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(82)90060-3"},{"key":"S0031182000064969_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(65)90062-7"},{"key":"S0031182000064969_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90688-6"},{"key":"S0031182000064969_ref014","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1986.tb09481.x"},{"key":"S0031182000064969_ref011","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(58)90094-5"},{"key":"S0031182000064969_ref007","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00026687"},{"key":"S0031182000064969_ref018","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a122866"},{"key":"S0031182000064969_ref009","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000064969","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,13]],"date-time":"2019-05-13T17:19:05Z","timestamp":1557767945000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000064969\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,8]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,8]]}},"alternative-id":["S0031182000064969"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000064969","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,8]]}}}