{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T03:24:32Z","timestamp":1770434672081,"version":"3.49.0"},"reference-count":34,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":8345,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1986,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The effects of malaria infection on RBC sialic acids and sialoglycoproteins were studied with asexual blood-stage infections of<jats:italic>Plasmodium knowlesi<\/jats:italic>in rhesus monkeys. Glycoprotein radio-isotope labelling methods were used to compare the sialoglycoproteins of normal RBC and<jats:italic>P. knowlesi<\/jats:italic>schizont-infected RBC (SI-RBC). Tritiation of glycoproteins from SI-RBC with the standard sialidase + galactose oxidase\/NaB<jats:sup>3<\/jats:sup>H<jats:sub>4<\/jats:sub>method or standard periodate\/NaB<jats:sup>3<\/jats:sup>H<jats:sub>4<\/jats:sub>method was significantly decreased when compared to normal RBC. However, tritium uptake into glycoproteins was normal when SI-RBC were treated with 5-fold higher concentrations of both enzymes in the first labelling method, or with a 5-fold increase in the molar ratio of periodate to sialic acid in the second method. The mobility of tritiated host cell glycoproteins on SDS\u2013polyacrylamide gels was identical for SI-RBC and normal RBC. New bands, possibly glycoproteins, of 230, 160, 90, 52, and 30 kDa were detected after labelling SI-RBC by the modified periodate\/NaB<jats:sup>3<\/jats:sup>H<jats:sub>4<\/jats:sub>method. Sialic acid analysis of normal rhesus monkey RBC (62\u03bcg\/10<jats:sup>10<\/jats:sup>RBC) revealed that 46% of the total sialic acid was<jats:italic>N<\/jats:italic>-glycolylneuraminic acid, 33% was<jats:italic>N<\/jats:italic>-acetyl-9-<jats:italic>O<\/jats:italic>-acetylneuraminic acid, and the remainder<jats:italic>N<\/jats:italic>-acetylneuraminic acid. SI-RBC collected either directly from infected monkeys or after<jats:italic>in vitro<\/jats:italic>culture of ring-infected RBC in horse serum, had increased total sialic acid (126 or 115\u03bcg\/10<jats:sup>10<\/jats:sup>RBC, respectively). The sialic acid content of infected RBC must increase during parasite development since RBC infected with ring-stage<jats:italic>P. knowlesi<\/jats:italic>had the same content as normal RBC. There was no significant difference in the ratio of the three sialic acids of SI-RBC and normal RBC. In contrast, the uninfected RBC from infected blood of different monkeys showed marked variation in sialic acid composition and generally had a lower sialic acid content than normal RBC.<\/jats:p>","DOI":"10.1017\/s0031182000065422","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:20:46Z","timestamp":1244218846000},"page":"527-543","source":"Crossref","is-referenced-by-count":10,"title":["Sialoglycoproteins and sialic acids of<i>Plasmodium knowlesi<\/i>schizont-infected erythrocytes and normal rhesus monkey erythrocytes"],"prefix":"10.1017","volume":"92","author":[{"given":"R. J.","family":"Howard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Reuter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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