{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T20:32:55Z","timestamp":1723062775160},"reference-count":35,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":4784,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1996,3]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>A<jats:italic>Leishmania infantum<\/jats:italic>cDNA library was screened with sera from dogs with viscero-cutaneous leishmaniasis. Sequence analysis of a positive clone isolated from the library revealed that it coded for the carboxyl-terminal region of a member of the 70-kDa heat-shock protein family. The full-length sequence of the<jats:italic>L. infantum<\/jats:italic>hsp70 gene was determined after isolation of genomic clones. This protein shows a high degree of sequence conservation with the homologous protein from other organisms. To test its antigenicity a recombinant Hsp70 protein fused to the maltose-binding protein was produced in<jats:italic>Escherichia coli<\/jats:italic>using the expression vector pMAL-cRI. By FAST-ELISA assays it was observed that while the complete recombinant protein was recognized by 100% of the sera, the 20 carboxyl-terminal amino acids of the protein were only recognized by 30% of those sera. Thus, although a B-cell epitope must be present within the carboxyl terminal end of the protein other antigenic determinant(s) must reside out of this region. The analysis of the cross-reactivity with mouse Hsp70 by Western blotting strongly suggests that the anti-Hsp70 antibodies generated by infection with<jats:italic>L. infantum<\/jats:italic>are directed at specific determinants of the<jats:italic>L. infantum<\/jats:italic>Hsp70. Thus, our results indicate that anti-Hsp70 autoantibodies are not induced during<jats:italic>Leishmania<\/jats:italic>infection.<\/jats:p>","DOI":"10.1017\/s0031182000065793","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T13:34:39Z","timestamp":1244208879000},"page":"277-284","source":"Crossref","is-referenced-by-count":26,"title":["During canine viscero-cutaneous leishmaniasis the anti-Hsp70 antibodies are specifically elicited by the parasite protein"],"prefix":"10.1017","volume":"112","author":[{"given":"L.","family":"Quijada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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