{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T09:10:04Z","timestamp":1710407404322},"reference-count":37,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":4692,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1996,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The detection of egg-derived antigens in the serum and urine of<jats:italic>Schistosoma mansoni<\/jats:italic>-infected individuals and experimental animals would provide an alternative method to assess the tissue egg burden. The detected levels are, however, not only a function of the amounts of antigen produced, but also of the processing or clearance by the host. In the present study the immunolocalization pattern of antigens using 2 recently described monoclonal antibodies to repetitive carbohydrate epitopes of<jats:italic>S. mansoni<\/jats:italic>soluble egg antigen (114\u20135B1\u2013A and 114\u20134D12\u2013A) in various organs of the host was investigated. In the liver strong immunoreactivity could be detected around the entrapped eggs and in egg-shells, as well as in Kupffer cells accumulating both antigen and schistosomal pigment. In the spleen, immunohistochemistry revealed antigen in the plasma as well as in secondary lysosomes of macrophages. Strong labelling was found in the vesicles of the eosinophilic granulocytes: indirect evidence perhaps for the presence of antigen\u2013antibody complexes. In conclusion, the secreted egg antigens were sequestered in the reticulo-endothelial macrophages of the liver and the spleen as already partly described for worm-derived antigens. The presence of large quantities of antigenic material in the spleen could suggest an important role of this organ in the clearance of antigen and might even provide an additional explanation for the hepatosplenomegaly mainly present in<jats:italic>S. mansoni<\/jats:italic>-infected children.<\/jats:p>","DOI":"10.1017\/s0031182000066117","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T13:30:06Z","timestamp":1244208606000},"page":"537-543","source":"Crossref","is-referenced-by-count":7,"title":["Immunohistochemical and ultrastructural localization of<i>Schistosoma mansoni<\/i>soluble egg antigens processed by the infected host"],"prefix":"10.1017","volume":"112","author":[{"given":"J. J. P. M.","family":"Bogers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. A. M.","family":"Nibbeling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Deelder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. A. E.","family":"Van Marck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000066117_ref017","doi-asserted-by":"publisher","DOI":"10.1007\/BF00926690"},{"key":"S0031182000066117_ref015","doi-asserted-by":"publisher","DOI":"10.1007\/BF00925485"},{"key":"S0031182000066117_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(76)90081-3"},{"key":"S0031182000066117_ref004","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000064702"},{"key":"S0031182000066117_ref003","article-title":"Quantitative and morphological aspects of Unicryl versus Lowicryl K4M embedding in immunoelectron microscopical studies","author":"Bogers","year":"1995","journal-title":"Journal of Histochemistry and Cytochemistry"},{"key":"S0031182000066117_ref034","first-page":"251","article-title":"Fibrosis around schistosomal egg antigen-coated beads in the liver of mice","volume":"7","author":"Van Marck","year":"1983","journal-title":"Contributions to Microbiology and Immunology"},{"key":"S0031182000066117_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(92)90144-Q"},{"key":"S0031182000066117_ref006","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1994.50.612"},{"key":"S0031182000066117_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(91)90446-6"},{"key":"S0031182000066117_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(87)90064-6"},{"key":"S0031182000066117_ref024","first-page":"639","article-title":"Advances in epidemiology survey methodology and techniques in schistosomiasis","volume":"58","author":"Mott","year":"1980","journal-title":"Bulletin of the World Health Organization"},{"key":"S0031182000066117_ref027","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1994.50.487"},{"key":"S0031182000066117_ref014","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1986.35.549"},{"key":"S0031182000066117_ref037","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1056\/NEJM199104183241607","article-title":"The immunobiology of eosinophils","volume":"324","author":"Weller","year":"1991","journal-title":"The New England Journal of Medicine"},{"key":"S0031182000066117_ref019","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(76)90011-0"},{"key":"S0031182000066117_ref005","doi-asserted-by":"publisher","DOI":"10.1007\/BF00931497"},{"key":"S0031182000066117_ref002","volume-title":"A Textbook of Histology","author":"Bloom","year":"1975"},{"key":"S0031182000066117_ref026","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.4049\/jimmunol.112.4.1500","article-title":"The characteristics of a circulating antigen in schistosomiasis","volume":"112","author":"Nash","year":"1974","journal-title":"Journal of Immunology"},{"key":"S0031182000066117_ref022","doi-asserted-by":"publisher","DOI":"10.1007\/BF00539458"},{"key":"S0031182000066117_ref018","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1990.42.347"},{"key":"S0031182000066117_ref032","first-page":"373","article-title":"Presence of the circulating polysaccharide antigen in the liver of mice infected with Schistosoma mansoni","volume":"55","author":"Van Marck","year":"1975","journal-title":"Annales de la Societe beige de M\u00e9decine tropicale"},{"key":"S0031182000066117_ref020","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1992.46.737"},{"key":"S0031182000066117_ref025","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1974.23.1085"},{"key":"S0031182000066117_ref031","first-page":"137","volume-title":"Basic and Clinical Immunology","author":"Terr","year":"1994"},{"key":"S0031182000066117_ref021","first-page":"397","article-title":"A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni","volume":"14","author":"Katz","year":"1972","journal-title":"Revista do Institute de medicina tropical de S\u00e3o Paulo"},{"key":"S0031182000066117_ref023","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000075995"},{"key":"S0031182000066117_ref028","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1994.50.585"},{"key":"S0031182000066117_ref001","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1967.16.606"},{"key":"S0031182000066117_ref029","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1967.16.473"},{"key":"S0031182000066117_ref030","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(82)90121-7"},{"key":"S0031182000066117_ref033","first-page":"1","article-title":"The glomerulopathy associated with Schistosoma mansoni infection. An experimental study in the mouse","volume":"50","author":"Van Marck","year":"1983","journal-title":"Ada Leidensia"},{"key":"S0031182000066117_ref036","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.4049\/jimmunol.135.2.1421","article-title":"Characterization of developmentally regulated epitopes of Schistosoma mansoni egg glycoprotein antigens","volume":"135","author":"Weiss","year":"1985","journal-title":"Journal of Immunology"},{"key":"S0031182000066117_ref035","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1974.23.1088"},{"key":"S0031182000066117_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(80)90004-1"},{"key":"S0031182000066117_ref008","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1989.40.268"},{"key":"S0031182000066117_ref007","doi-asserted-by":"crossref","first-page":"2442","DOI":"10.4049\/jimmunol.124.5.2442","article-title":"Purification, immunochemical, and biologic characterization of the Schistosoma circulating M antigen","volume":"124","author":"Carlier","year":"1980","journal-title":"Journal of Immunology"},{"key":"S0031182000066117_ref011","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(88)90523-8"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000066117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T08:57:31Z","timestamp":1710406651000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000066117\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,6]]},"references-count":37,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1996,6]]}},"alternative-id":["S0031182000066117"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000066117","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,6]]}}}