{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T03:53:45Z","timestamp":1770695625924,"version":"3.49.0"},"reference-count":15,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":4570,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1996,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Intraperitoneal implantation of adult gravid females of the bovine filarial parasite,<jats:italic>Setaria digitata<\/jats:italic>in<jats:italic>Mastomys coucha<\/jats:italic>was found to induce microfilaraemia lasting for about 125 days. The microfilariae (mf) could be detected as early as 4 days postimplantation (p.i.) and peak levels of about 30 mf in 20 \u00b5l of blood were observed by 21 days. A significant positive correlation was found between mf density and the body weight of recipients pre-implantation. The implanted adult worms were generally viable only for less than 1 week. Implantation resulted in a significant decrease in total leucocytes and erythrocytes, induction of eosinophilia, splenomegaly and anti-erythrocyte autoantibodies. The microfilariae in circulation developed into 3rd-stage infective larvae (L<jats:sub>3<\/jats:sub>) when fed onto<jats:italic>Aedes aegypti<\/jats:italic>(ref<jats:sup>m<\/jats:sup>, Liverpool strain). The mf in circulation were found to be eliminated by oral administration of diethylcarbamazine citrate, indicating the usefulness of this model for screening potential anti-microfilarial drugs. During the microfilaraemic phase, priming with tetanus toxoid (TT) resulted in significantly decreased production of anti-toxin levels indicating a state of generalized immunosuppression. Induction of antibodies to various fractionated antigenic components of adult parasites could be demonstrated by enzyme immunoassay (EIA) in<jats:italic>M. coucha<\/jats:italic>implanted with live or cold-stunned adult worms. The<jats:italic>S. digitata\u2013M. coucha<\/jats:italic>model thus is found amenable to perform chemotherapeutic and immunobiological investigations in experimental filariasis.<\/jats:p>","DOI":"10.1017\/s0031182000066488","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T13:23:51Z","timestamp":1244208231000},"page":"323-330","source":"Crossref","is-referenced-by-count":12,"title":["<i>Setaria digitata<\/i>microfilaraemia in<i>Mastomys coucha<\/i>: an animal model for chemotherapeutic and immunobiological studies"],"prefix":"10.1017","volume":"113","author":[{"given":"S.","family":"Mukhopadhyay","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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