{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T04:28:17Z","timestamp":1712896097874},"reference-count":38,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":4753,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1996,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Polymerase chain reaction (PCR) combined with non-radioactive DNA hybridization was applied for the detection and characterization of a 150 bp tandem repeat of <jats:italic>Onchocerca volvulus<\/jats:italic>. DNA of worms from western Uganda was amplified and then probed with a digoxygenin-labelled oligonucleotide, specific for the forest form of <jats:italic>O. volvulus<\/jats:italic> and compared to samples from various African countries. Hybridization was only observed with PCR products from the forest in Liberia, south-eastern Ghana, Benin and southern Cameroon, but not with worms from Uganda or the savannah in Burkina Faso and northern Ghana. A nested PCR using primers derived form the forest form-specific DNA sequence confirmed these results. Morphometric studies revealed length differences between the microfilariae of Ugandan <jats:italic>O. volvulus<\/jats:italic> to those of West Africa, especially to those of the savannah in Burkina Faso. It is concluded that the forest\/savannah classification of <jats:italic>O. volvulus<\/jats:italic> from West Africa is not suitable for <jats:italic>Simulium weasel<\/jats:italic>-transmitted <jats:italic>O. volvulus<\/jats:italic> from Uganda.<\/jats:p>","DOI":"10.1017\/s0031182000066634","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T09:34:23Z","timestamp":1244194463000},"page":"401-408","source":"Crossref","is-referenced-by-count":20,"title":["Strain differentiation of <i>Onchocerca volvulus<\/i> from Uganda using DNA probes"],"prefix":"10.1017","volume":"112","author":[{"given":"P.","family":"Fischer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Bamuhiiga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. H. D.","family":"Kilian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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