{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T09:43:10Z","timestamp":1710409390109},"reference-count":39,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":5819,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1993,5]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>A low molecular weight (15 kDa) surface antigen of the cattle filarial nematode,<jats:italic>Setaria digitata<\/jats:italic>, was earlier shown to be specifically recognized by the antibodies from human bancroftian filarial (Mf positive) patients' sera (Theodore &amp; Kaliraj, 1990). The filarial specific antibodies bound to a 15 kDa peptide in preparative Western blots were eluted and employed in screening of candidate antigens expressed in the genomic library of<jats:italic>Wuchereria bancrofti<\/jats:italic>at the IgG<jats:sub>4<\/jats:sub>subclass antibody level. A recombinant clone (\u03bb WbG7) reacting strongly with filarial sera but poorly with sera from patients infected with non-filarial helminths was selected for further studies. The 2 kb DNA insert of the clone \u03bb WbG7 was recloned into a pMAL vector and the recombinant clone pGT7 thus obtained was over-expressed and affinity purified. The purified 105 kDa fusion protein of the clone pGT7 was specific and was not recognized by the non-filarial sera at the IgG<jats:sub>4<\/jats:sub>level. All microfilaraemic individuals were positive by IgG<jats:sub>4<\/jats:sub>assay. However, similar attempts to diagnose by filarial-specific IgE assays failed to recognize microfilaraemic individuals. Moreover, by filarial-specific IgG<jats:sub>4<\/jats:sub>assays, the endemic normals were distinctly divided into two groups, showing higher and lower recognition for this antigen indicating current and past\/no infection. Among the filarial-IgG<jats:sub>4<\/jats:sub>(assay)-positive \u2018endemic normals\u2019, 14% showed \u2018microfilariae\u2019 during repeated peripheral night blood examination, confirming the validity of the recombinant antigen, pGT7 based assay.<\/jats:p>","DOI":"10.1017\/s0031182000067160","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:12:36Z","timestamp":1244211156000},"page":"413-420","source":"Crossref","is-referenced-by-count":10,"title":["Cloning, over-expression and evaluation of a recombinant fusion protein of<i>Wuchereria bancrofti<\/i>towards its application as a diagnostic agent for bancroftian filariasis"],"prefix":"10.1017","volume":"106","author":[{"given":"J. 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