{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:09:00Z","timestamp":1758632940594},"reference-count":49,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":5635,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1993,11]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Adhesion of parasitized red blood cells to vascular endothelium is thought to play an important role in the development of the ischaemic complications associated with severe falciparum malaria. Using a novel, flow-based assay, we have investigated the adhesion of parasitized red blood cells to formalin-fixed human umbilical vein endothelial cells (HUVEC), for isolates obtained from 32 Gambian subjects with mild or severe falciparum malaria. Red cells infected with wild strains of<jats:italic>Plasmodium falciparum<\/jats:italic>were able to adhere to HUVEC under physiologically relevant flow conditions, but the level of adhesion was highly variable, ranging from 1 to 688 adherent cells per mm<jats:sup>2<\/jats:sup>of HUVEC. Within isolates, some adherent parasitized cells remained stationary, whilst other formed less stable interactions and rolled slowly over the cell surface. There was no significant difference in adhesion of parasitized cells between isolates obtained from mild or severe cases of malaria, although a subset of isolates did show very high levels of adhesion. The results suggest that there is not a simple relationship between the adhesion of parasitized cells to cultured endothelial cells (presumably via the receptor ICAM-1) and the clinical severity of the disease, although variation in microvascular adhesion<jats:italic>in vivo<\/jats:italic>may still be a determinant of ischaemic complications.<\/jats:p>","DOI":"10.1017\/s0031182000067706","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:06:12Z","timestamp":1244210772000},"page":"359-368","source":"Crossref","is-referenced-by-count":18,"title":["Adhesion of parasitized red blood cells to cultured endothelial cells: a flow-based study of isolates from Gambian children with falciparum malaria"],"prefix":"10.1017","volume":"107","author":[{"given":"B. M.","family":"Cooke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Morris-Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. M.","family":"Greenwood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. B.","family":"Nash","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000067706_ref009","doi-asserted-by":"publisher","DOI":"10.1038\/341057a0"},{"key":"S0031182000067706_ref049","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(86)90407-4"},{"key":"S0031182000067706_ref045","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1992.46.503"},{"key":"S0031182000067706_ref047","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198202113060601"},{"key":"S0031182000067706_ref043","doi-asserted-by":"publisher","DOI":"10.1126\/science.781840"},{"key":"S0031182000067706_ref038","doi-asserted-by":"publisher","DOI":"10.1038\/357689a0"},{"key":"S0031182000067706_ref039","doi-asserted-by":"publisher","DOI":"10.1038\/318064a0"},{"key":"S0031182000067706_ref037","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.11.3829"},{"key":"S0031182000067706_ref033","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.4.1183"},{"key":"S0031182000067706_ref036","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1991.44.168"},{"key":"S0031182000067706_ref032","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/164.1.163"},{"key":"S0031182000067706_ref031","first-page":"342a","article-title":"Roles of different receptors in cytoadhesion of red cells parasitized by Plasmodium falciparum","volume":"80","author":"Nash","year":"1992","journal-title":"Blood"},{"key":"S0031182000067706_ref046","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.5.1835"},{"key":"S0031182000067706_ref042","doi-asserted-by":"crossref","first-page":"78","DOI":"10.4049\/jimmunol.148.1.78","article-title":"Human dermal microvascular endothelial but not human umbilical vein endothelial cells express CD36 in vivo and in vitro","volume":"148","author":"Swerlick","year":"1992","journal-title":"Journal of Immunology"},{"key":"S0031182000067706_ref026","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(88)90123-3"},{"key":"S0031182000067706_ref017","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1182\/blood.V76.9.1845.1845","article-title":"In vitro rosetting, cytoadherence, and microagglutination properties of Plasmodium falciparum-infected erythrocytes from Gambian and Tanzanian patients","volume":"76","author":"Hasler","year":"1990","journal-title":"Blood"},{"key":"S0031182000067706_ref025","first-page":"385","article-title":"Human cerebral malaria. A quantitative ultrastructural analysis of parasitized erythrocyte sequestration","volume":"119","author":"Macpherson","year":"1985","journal-title":"American Journal of Pathology"},{"key":"S0031182000067706_ref040","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1182\/blood.V71.1.71.71","article-title":"Thrombospondin mediates the cytoadherence of Plasmodium falciparum-infected red cells to vascular endothelium in shear flow conditions","volume":"71","author":"Rock","year":"1988","journal-title":"Blood"},{"key":"S0031182000067706_ref024","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1971.20.655"},{"key":"S0031182000067706_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(89)90190-2"},{"key":"S0031182000067706_ref010","doi-asserted-by":"publisher","DOI":"10.1084\/jem.171.6.1883"},{"key":"S0031182000067706_ref030","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1992.tb06955.x"},{"key":"S0031182000067706_ref007","doi-asserted-by":"crossref","first-page":"3494","DOI":"10.4049\/jimmunol.135.5.3494","article-title":"Monoclonal antibody OKM5 inhibits the in vitro binding of Plasmodium falciparum-infected erythrocytes to monocytes, endothelial, and C32 melanoma cells","volume":"135","author":"Barnwell","year":"1985","journal-title":"Journal of Immunology"},{"key":"S0031182000067706_ref023","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.43.5.738"},{"key":"S0031182000067706_ref018","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1128\/IAI.59.6.2135-2139.1991","article-title":"Rosette formation of Plasmodium falciparum-infected erythrocytes from patients with acute malaria","volume":"59","author":"Ho","year":"1991","journal-title":"Infection and Immunity"},{"key":"S0031182000067706_ref004","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1182\/blood.V70.1.152.152","article-title":"Endothelial cell interactions with sickle cell, sickle trait, mechanically injured, and normal erythrocytes under controlled flow","volume":"70","author":"Barabino","year":"1987","journal-title":"Blood"},{"key":"S0031182000067706_ref019","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1128\/IAI.59.3.873-878.1991","article-title":"Clinical correlates of in vitro P. falciparum cytoadherence","volume":"59","author":"Ho","year":"1991","journal-title":"Infection and Immunity"},{"key":"S0031182000067706_ref013","doi-asserted-by":"publisher","DOI":"10.1006\/mvre.1993.1004"},{"key":"S0031182000067706_ref048","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1991.45.578"},{"key":"S0031182000067706_ref001","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1988.39.3"},{"key":"S0031182000067706_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(87)90170-2"},{"key":"S0031182000067706_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(90)90184-6"},{"key":"S0031182000067706_ref002","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1990.43.30"},{"key":"S0031182000067706_ref003","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1972.sp009808"},{"key":"S0031182000067706_ref035","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)92903-U"},{"key":"S0031182000067706_ref012","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-4285-1_5"},{"key":"S0031182000067706_ref044","first-page":"883","article-title":"The fine structure of Plasmodium falciparum and its host erythrocytes in natural malarial infections in man","volume":"35","author":"Trager","year":"1986","journal-title":"Bulletin of the World Health Organization"},{"key":"S0031182000067706_ref034","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90406-6"},{"key":"S0031182000067706_ref022","first-page":"H481","article-title":"Microvascular haematocrit and red cell flow in resting and contracting striated muscle","volume":"237","author":"Klitzman","year":"1979","journal-title":"American Journal of Physiology"},{"key":"S0031182000067706_ref011","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)93174-N"},{"key":"S0031182000067706_ref029","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1182\/blood.V79.3.798.798","article-title":"Rheological analysis of the adhesive interactions of red blood cells parasitized by Plasmodium falciparum","volume":"79","author":"Nash","year":"1992","journal-title":"Blood"},{"key":"S0031182000067706_ref020","doi-asserted-by":"crossref","first-page":"2603","DOI":"10.1182\/blood.V74.8.2603.2603","article-title":"Molecular studies related to the pathogenesis of cerebral malaria","volume":"74","author":"Howard","year":"1989","journal-title":"Blood"},{"key":"S0031182000067706_ref006","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114234"},{"key":"S0031182000067706_ref014","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.16.5075"},{"key":"S0031182000067706_ref015","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1992.tb08249.x"},{"key":"S0031182000067706_ref021","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.2.641"},{"key":"S0031182000067706_ref041","doi-asserted-by":"publisher","DOI":"10.1016\/0248-4900(92)90022-S"},{"key":"S0031182000067706_ref028","first-page":"441","article-title":"Clinical features and prognostic indicators in paediatric cerebral malaria: a study of 131 comatose Malawian children","volume":"265","author":"Molyneux","year":"1989","journal-title":"Quarterly Journal of Medicine"},{"key":"S0031182000067706_ref027","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.1972.tb00001.x"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000067706","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T14:30:39Z","timestamp":1685025039000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000067706\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,11]]},"references-count":49,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1993,11]]}},"alternative-id":["S0031182000067706"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000067706","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,11]]}}}