{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T18:06:47Z","timestamp":1750442807964},"reference-count":12,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":5543,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1994,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The transport of [<jats:sup>14<\/jats:sup>C]glucose by <jats:italic>Hymenolepis microstoma in vitro<\/jats:italic> following <jats:italic>in vivo<\/jats:italic> treatment with cyclosporin A (CsA) was determined over a range of concentrations. For untreated (control) worms glucose uptake showed saturation kinetics with a small diffusion component. Estimates of the maximum velocity of glucose uptake (<jats:italic>V<jats:sub>max<\/jats:sub><\/jats:italic>) and the affinity of substrate for the glucose transporter (<jats:italic>K<jats:sub>t<\/jats:sub><\/jats:italic>) revealed that untreated 8-day-old worms had a <jats:italic>V<jats:sub>max<\/jats:sub><\/jats:italic> twice that of 15-day-old worms and that younger worms had a lower <jats:italic>K<jats:sub>t<\/jats:sub><\/jats:italic>. An inverse relationship was demonstrated between log<jats:sub>10<\/jats:sub> worm weight and the rate of uptake of [<jats:sup>14<\/jats:sup>C]glucose, reflecting the relatively greater number of glucose transporters due to the larger surface area: volume ratio of smaller worms. Treatment of <jats:italic>H. microstoma<\/jats:italic> with CsA <jats:italic>in vivo<\/jats:italic> significantly increased the diffusion component of glucose uptake <jats:italic>in vitro<\/jats:italic>. Parasites from drug-treated mice had a significantly lower <jats:italic>V<jats:sub>max<\/jats:sub><\/jats:italic> for glucose uptake than size-matched controls. The affinity of glucose for its transporter in CsA-treated worms (<jats:italic>K<jats:sub>t<\/jats:sub><\/jats:italic>) was not significantly different from size-matched controls. Both juvenile and adult worms underwent transient depletion in total glycogen content after CsA treatment <jats:italic>in vivo<\/jats:italic>. The data confirm that CsA treatment <jats:italic>in vivo<\/jats:italic> disrupts the functional integrity of the worm tegument, one facet of which is impaired acquisition of glucose.<\/jats:p>","DOI":"10.1017\/s0031182000068323","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:01:41Z","timestamp":1244210501000},"page":"223-228","source":"Crossref","is-referenced-by-count":9,"title":["Cyclosporin A: drug treatment <i>in vivo<\/i> affects the kinetics of [<sup>14<\/sup>C]glucose transport in <i>Hymenolepis microstoma in vitro<\/i>"],"prefix":"10.1017","volume":"108","author":[{"given":"J. M.","family":"Wastling","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. H.","family":"Chappell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000068323_ref012","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000063794"},{"key":"S0031182000068323_ref011","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-058980-7.50010-5"},{"key":"S0031182000068323_ref010","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1963.tb40664.x"},{"key":"S0031182000068323_ref006","first-page":"273","article-title":"Graphical analysis of single enzyme systems","volume":"17","author":"Hofstee","year":"1956","journal-title":"Enzymologia"},{"key":"S0031182000068323_ref003","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000086029"},{"key":"S0031182000068323_ref002","first-page":"5","volume-title":"Cyclosporin A. Proceedings of an International Conference on Cyclosporin A","author":"Borel","year":"1982"},{"key":"S0031182000068323_ref001","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000085991"},{"key":"S0031182000068323_ref008","doi-asserted-by":"publisher","DOI":"10.2307\/3279320"},{"key":"S0031182000068323_ref004","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000062211"},{"key":"S0031182000068323_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(75)90016-8"},{"key":"S0031182000068323_ref005","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000075338"},{"key":"S0031182000068323_ref007","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000060078"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000068323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,15]],"date-time":"2019-05-15T19:22:54Z","timestamp":1557948174000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000068323\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2]]},"references-count":12,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,2]]}},"alternative-id":["S0031182000068323"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000068323","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2]]}}}