{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T17:59:22Z","timestamp":1760983162069},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":6092,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1992,8]]},"abstract":"<jats:p>This study was undertaken to assess whether glycolipid antigens (particularly gangliosides) are associated with<jats:italic>Pneumocystis carinii<\/jats:italic>obtained from human lungs. Gangliosides were extracted, purified in high performance thin-layer chromatography and stained with resorcinol. Two resorcinol-positive bands, co-migrating with GM<jats:sub>1<\/jats:sub>and GD<jats:sub>1a<\/jats:sub>were demonstrated, suggesting the existence of ganglioside molecules on<jats:italic>P. carinii<\/jats:italic>. No resorcinol-positive bands were revealed in the pulmonary control tissue. In addition, an antiserum obtained from rabbits immunized with<jats:italic>P. carinii<\/jats:italic>antigen reacted with gangliosides GM<jats:sub>1<\/jats:sub>, and GD<jats:sub>1a<\/jats:sub>, as revealed by a dot immunobinding assay. This reactivity was inhibited by first incubating the antiserum with ganglioside micelles. Furthermore, anti-glycosphingolipid antibodies (aGM<jats:sub>1<\/jats:sub>) reacted with the bands of 200 and 55 kDa of<jats:italic>P. carinii<\/jats:italic>antigen. These results suggest that ganglioside antigens expressed on<jats:italic>P. carinii<\/jats:italic>can trigger specific immune responses.<\/jats:p>","DOI":"10.1017\/s0031182000073613","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:19:38Z","timestamp":1244211578000},"page":"1-6","source":"Crossref","is-referenced-by-count":12,"title":["Evidence for the existence of ganglioside molecules on<i>Pneumocystis carinii<\/i>from human lungs"],"prefix":"10.1017","volume":"105","author":[{"given":"M.","family":"Sorice","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Lenti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Misasi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Contini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Cignarella","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Griggi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Vullo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Masala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000073613_ref007","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a123016"},{"key":"S0031182000073613_ref002","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipids from animal tissues","volume":"226","author":"Folch","year":"1957","journal-title":"Journal of Biological Chemistry"},{"key":"S0031182000073613_ref012","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1128\/IAI.55.3.736-741.1987","article-title":"Phospholipid profile of Pneumnocystis carinii and its interaction with alveolar type II epithelial cells","volume":"55","author":"Pesanti","year":"1987","journal-title":"Infection and Immunity"},{"key":"S0031182000073613_ref006","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-2516-9_3"},{"key":"S0031182000073613_ref009","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"S0031182000073613_ref003","doi-asserted-by":"publisher","DOI":"10.1021\/bi00573a028"},{"key":"S0031182000073613_ref004","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/158.4.848"},{"key":"S0031182000073613_ref017","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1128\/IAI.57.5.1363-1368.1989","article-title":"Glycoproteins composed of major surface immunodeterminants of Pneumocystis carinii","volume":"57","author":"Tanabe","year":"1989","journal-title":"Infection and Immunity"},{"key":"S0031182000073613_ref018","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"S0031182000073613_ref005","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1128\/IAI.51.1.125-133.1986","article-title":"Development and characterization of monoclonal antibodies to Pneumocystis carinii","volume":"51","author":"Graves","year":"1986","journal-title":"Infection and Immunity"},{"key":"S0031182000073613_ref019","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(79)90088-2"},{"key":"S0031182000073613_ref011","volume-title":"Serodiagnostic des affection \u00e0 Pneumocystis carinii. 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