{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T08:58:37Z","timestamp":1788339517991,"version":"build-2803163510"},"reference-count":38,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":6092,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1992,8]]},"abstract":"<jats:p>Praziquantel is widely used for treatment of schistosomiasis. Elimination of intravascular worms involves a combination of drug-induced changes at the worm surface and a host immune response against exposed parasite components. Loss of spines is a typical feature of schistosomes obtained from praziquantel-treated infected animals. The mechanism for this is unknown. We have considered the possibility that praziquantel treatment involves exposure of surface spines consisting of \u2018paracrystalline\u2019 actin and a subsequent host response against actin through mechanisms established in the host independently from schistosomal infection. Drug-induced exposure of actin was demonstrated by fluorescence microscopy using anti-actin antibodies and phallacidin, an actin-binding mushroom toxin. Actin spines remained intact at the schistosome surface after<jats:italic>in vitro<\/jats:italic>exposure, but spine morphology was lost after<jats:italic>in vivo<\/jats:italic>exposure to praziquantel. Disintegration of spines<jats:italic>in vivo<\/jats:italic>was associated with binding of host antibodies.<jats:italic>In vitro<\/jats:italic>spine destruction could be seen in the presence of normal human serum. The effect was linked to calcium-dependent binding of actin depolymerizing factor, gelsolin.<\/jats:p>","DOI":"10.1017\/s0031182000073704","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:19:38Z","timestamp":1244211578000},"page":"71-79","source":"Crossref","is-referenced-by-count":25,"title":["<i>Schistosoma mansoni<\/i>: praziquantel-induced tegumental lesion exposes actin of surface spines and allows binding of actin depolymerizing factor, 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