{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T14:16:52Z","timestamp":1773238612438,"version":"3.50.1"},"reference-count":22,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":6092,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1992,8]]},"abstract":"<jats:p>An enzyme-linked immunosorbent assay (ELISA) using crude worm extracts (CWE) and mixtures of these as antigens of five Spanish isolates (P, C, B1, B2 and W) was developed for detecting homologous and heterologous experimental infections with these isolates between \u2013 14 and 82 days post-infection (p.i.) in white and Iberian pigs. A total of 243 pigs (Ilberian or cross-bred with this race) with numerous parasitic infections were also screened for the presence of antibodies to a mixture of CWE of C, B1 and B2 isolate. The test showed a specificity of 93\u00b71\u201398\u00b79% depending on the cut-off values and a maximum sensitivity of 92\u00b78\u2013100% between days 34 and 82 p.i. A low grade of infectivity was shown in the T3 isolates compared to the T1 isolates (P, C, B1 and B2) but high cross-reactions were observed between all the isolates with minor differences between P and W isolates. The highest antibody response was found in P infections and the lowest in pigs infected with the W isolate. A clear association between the presence of several parasitic infections and false positive reactions was not found, but an important relation was shown between high background levels and the Iberian race in experimentally and conventionally raised pigs<\/jats:p>","DOI":"10.1017\/s0031182000073753","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:19:38Z","timestamp":1244211578000},"page":"111-115","source":"Crossref","is-referenced-by-count":10,"title":["<i>Trichinella<\/i>strain, pig race and other parasitic infections as factors in the reliability of ELISA for the detection of swine trichinellosis"],"prefix":"10.1017","volume":"105","author":[{"given":"F.","family":"Serrano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Reina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Navarrete","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000073753_ref009","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.2460\/ajvr.1986.47.05.1046","article-title":"Field evaluation of the enzyme-linked immunosorbent assay for swine trichinosis: efficacy of the excretory\u2013secretory antigen","volume":"47","author":"Murreli","year":"1986","journal-title":"American Journal of Veterinary Research"},{"key":"S0031182000073753_ref017","unstructured":"Van Knapen F. & Franchimont J. H. (1984). Further studies with sera from Trichinella spiralis free pigs from 10 European countries and Canada to determine the detection levels for the enzyme-linked immunosorbent assay (ELISA). Report on behalf of members of the EEC working group on trichinellosis, January 1984."},{"key":"S0031182000073753_ref002","first-page":"182","volume-title":"Trichinellosis","author":"Arriaga","year":"1989"},{"key":"S0031182000073753_ref020","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(80)90067-9"},{"key":"S0031182000073753_ref022","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.1979.9693741"},{"key":"S0031182000073753_ref013","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1983.32.1275"},{"key":"S0031182000073753_ref012","first-page":"487","volume-title":"Trichinellosis","author":"Ruitenberg","year":"1978"},{"key":"S0031182000073753_ref018","first-page":"399","volume-title":"Trichinellosis","author":"Van Knapen","year":"1981"},{"key":"S0031182000073753_ref004","first-page":"133","article-title":"Detection of antibodies to Trichinella spiralis in farm pigs","volume":"57","author":"\u010cuperlovi\u0107","year":"1987","journal-title":"Veterinarski Archiv"},{"key":"S0031182000073753_ref007","first-page":"262","volume-title":"Trichinellosis","author":"Isenstein","year":"1989"},{"key":"S0031182000073753_ref015","first-page":"497","article-title":"Comparative assessment of a double antibody enzyme immunoassay test kit and a triple antibody enzyme immunoassay for the diagnosis of Trichinella spiralis spiralis and Trichinella spiralis nativa infections in swine","volume":"53","author":"Smith","year":"1989","journal-title":"Canadian Journal of Veterinary Research"},{"key":"S0031182000073753_ref001","first-page":"102","volume-title":"Trichinellosis","author":"Armas Serra","year":"1989"},{"key":"S0031182000073753_ref011","first-page":"747","article-title":"ELISA (enzyme-linked immunosorbent assay) as a preventive and representative control method for the detection of Trichinella spiralis infections in slaughter pigs","volume":"21","author":"Ruitenberg","year":"1975","journal-title":"Wiadomosci Parazytologiczne"},{"key":"S0031182000073753_ref008","first-page":"96","volume-title":"Trichinellosis","author":"Mart\u00ednez Fern\u00e1ndez","year":"1989"},{"key":"S0031182000073753_ref019","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(84)90017-7"},{"key":"S0031182000073753_ref003","first-page":"1558","article-title":"Identification and distribution of swine serum immunoglobulins that react with Trichinella spiralis antigens and may interfere with the enzyme-labeled antibody test for trichinosis","volume":"40","author":"Clinard","year":"1979","journal-title":"American Journal of Veterinary Research"},{"key":"S0031182000073753_ref005","first-page":"480","article-title":"M\u00f6glichkeiten des Nachweises und der Differenzierung von zwei Sarcocystis Arten des Schweines","volume":"90","author":"Erber","year":"1977","journal-title":"Berliner und M\u00fcnchener Tier\u00e4rztliche Wochenschrift"},{"key":"S0031182000073753_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(83)90051-1"},{"key":"S0031182000073753_ref010","first-page":"76","volume-title":"Trichinellosis","author":"Pozio","year":"1989"},{"key":"S0031182000073753_ref014","first-page":"413","article-title":"Detection of Trichinella spiralis nativa antibodies in porcine sera by ELISA using T. spiralis spiralis excretory\u2013secretory antigen","volume":"51","author":"Smith","year":"1987","journal-title":"Canadian Journal of Veterinary Research"},{"key":"S0031182000073753_ref016","doi-asserted-by":"publisher","DOI":"10.2307\/3282561"},{"key":"S0031182000073753_ref021","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(84)90004-9"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000073753","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T23:22:47Z","timestamp":1739143367000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000073753\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,8]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,8]]}},"alternative-id":["S0031182000073753"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000073753","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,8]]}}}