{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T04:57:14Z","timestamp":1698296234188},"reference-count":22,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":5970,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1992,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The survival, growth and development of <jats:italic>Onchocerca lienalis<\/jats:italic> 3rd-stage (L3) larvae implanted into mice within micropore chambers has been studied with a view to developing a vaccination model for studies of protective immunity in onchocerciasis. The influence of host genetics on worm recoveries and development (growth and moulting rate) was analysed in a panel of inbred mice (CBA, BALB\/c, DBA\/2, SJL, 129J, C57BL\/10 (B10), C3H\/He and NIH), together with mice of BALB and B10 backgrounds with different major histocompatibility complex (H-2) genes (BALB\/c, BALB.K, BALB.B and B10, B10.D2\/n, B10.BR, B10.S). Parasite recoveries and development were similar in all mouse genotypes tested. They were unaffected by procedures designed to block or modulate phagocytic cell function with carbon or carrageenan, or to suppress inflammation by treatment with hydrocortisone acetate. A comparison of chambers sealed with membranes designed to admit (5\u00b70 \u03bcm pore size) or exclude (0\u00b72 \u03bcm pore size) host cells demonstrated no effect on the percentage recovery of living larvae, although dead larvae were more frequently retrieved when cells were excluded. Recoveries and rates of development of larvae implanted into immunodeficient <jats:italic>scid<\/jats:italic> mice and athymic Hooded rats were similar to those recorded in immunocompetent controls. We conclude that host genetic factors and immunocompetence are not significant determinants of survival, growth or development of <jats:italic>O. lienalis<\/jats:italic> larvae implanted within micropore chambers into naive mice. Despite its limitations, the use of this system merits further investigation as an approach to the study of protective immunity against developing larvae in onchocerciasis.<\/jats:p>","DOI":"10.1017\/s003118200007462x","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:17:43Z","timestamp":1244211463000},"page":"445-451","source":"Crossref","is-referenced-by-count":4,"title":["Host strain, H-2 genotype and immunocompetence do not affect the survival or development of <i>Onchocerca lienalis<\/i> infective larvae implanted within micropore chambers into mice or rats"],"prefix":"10.1017","volume":"105","author":[{"given":"M. J.","family":"Taylor","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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E.","family":"Bianco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S003118200007462X_ref003","doi-asserted-by":"publisher","DOI":"10.2307\/3282456"},{"key":"S003118200007462X_ref002","first-page":"165","article-title":"Protective immune responses of the jird to larval Dipetalonema viteae","volume":"57","author":"Abraham","year":"1986","journal-title":"Immunology"},{"key":"S003118200007462X_ref022","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00027814"},{"key":"S003118200007462X_ref007","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00009019"},{"key":"S003118200007462X_ref014","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.3.659"},{"key":"S003118200007462X_ref018","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1985.tb00081.x"},{"key":"S003118200007462X_ref011","doi-asserted-by":"publisher","DOI":"10.1007\/BF00927088"},{"key":"S003118200007462X_ref021","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000055268"},{"key":"S003118200007462X_ref010","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1983.tb00746.x"},{"key":"S003118200007462X_ref019","first-page":"120","article-title":"Development of infective larvae of Onchocerca volvulus in diffusion chambers implanted into Mastomys natalensis","volume":"36","author":"Strote","year":"1985","journal-title":"Tropical Medicine and Parasitology"},{"key":"S003118200007462X_ref016","first-page":"275","article-title":"Rodent models of filariasis","volume":"12","author":"Philipp","year":"1984","journal-title":"Contemporary Topics in Immunobiology"},{"key":"S003118200007462X_ref015","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.42.100188.003345"},{"key":"S003118200007462X_ref005","first-page":"138","volume-title":"Parasitic Worms, Zoonoses and Human Health in Africa","author":"Bianco","year":"1991"},{"key":"S003118200007462X_ref017","first-page":"292","article-title":"Protective immune responses with trickle infections of third-stage filarial larvae of Wuchereria bancrofti in mice","volume":"78","author":"Rajasekariah","year":"1989","journal-title":"Clinical and Experimental Immunology"},{"key":"S003118200007462X_ref001","doi-asserted-by":"publisher","DOI":"10.2307\/3282833"},{"key":"S003118200007462X_ref012","first-page":"317","article-title":"Attempts to establish Onchocerca volvulus infection in primates and small laboratory animals","volume":"39","author":"Kozek","year":"1982","journal-title":"Acta Tropica"},{"key":"S003118200007462X_ref013","first-page":"75","article-title":"Effect of macrophage blockade on the resistance to a primary Brugia pahangi infection of female BALB\/c mice","volume":"40","author":"Nakanishi","year":"1989","journal-title":"Tropical Medicine and Parasitology"},{"key":"S003118200007462X_ref006","first-page":"57","article-title":"A semi-automated system of intrathoracic injection for the large-scale production of Onchocerca lienalis infective larvae","volume":"40","author":"Bianco","year":"1989","journal-title":"Tropical Medicine and Parasitology"},{"key":"S003118200007462X_ref004","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1989.40.598"},{"key":"S003118200007462X_ref008","first-page":"29","article-title":"Development of Dirofilaria immitis third stage larvae (Nematoda: Filarioidea) in micropore chambers implanted into surrogate hosts","volume":"36","author":"Delves","year":"1985","journal-title":"Tropical Medicine and Parasitology"},{"key":"S003118200007462X_ref020","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00007380"},{"key":"S003118200007462X_ref009","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1991.44.151"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S003118200007462X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,16]],"date-time":"2019-05-16T20:06:25Z","timestamp":1558037185000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S003118200007462X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,12]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1992,12]]}},"alternative-id":["S003118200007462X"],"URL":"https:\/\/doi.org\/10.1017\/s003118200007462x","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,12]]}}}