{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T12:56:00Z","timestamp":1761310560790},"reference-count":22,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":5362,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1994,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The recombinant<jats:italic>Echinococcus multilocularis<\/jats:italic>antigen ll\/3\u201310 is one of the most promising tools for immunodiagnosis of alveolar echinococcosis in human patients. Its nucleic acid sequence represents a part of the<jats:italic>E. multilocularis<\/jats:italic>gene encoding the metacestode antigen II\/3, the former being basically present and expressed in both<jats:italic>E. multilocularis<\/jats:italic>and<jats:italic>E. granulosus<\/jats:italic>. Most (94%) patients with alveolar echinococcosis respond to infection with a marked anti-II\/3\u201310 IgG synthesis; in contrast, most of the cystic echinococcosis patients do not, for some reason, recognize the recombinant antigen. We tackled this problem by generating cDNA derived from both<jats:italic>E. granulosus<\/jats:italic>and<jats:italic>E. multilocularis<\/jats:italic>full length II\/3 genes, performed by reverse transcription and PCR amplification. Sequence analysis revealed a very high degree of conservation of the primary sequence of the antigen II\/3 in both<jats:italic>Echinococcus<\/jats:italic>species. cDNA fragments were subcloned and expressed in<jats:italic>E. coli<\/jats:italic>as fusion proteins with<jats:italic>Schistosoma japonicum<\/jats:italic>glutathione S-transferase. Recombinant proteins were affinity purified and comparatively assessed by ELISA with respect to antibody-binding characteristics. Sera from patients suffering from cystic echinococcosis showed no significant differences in reactivity with the antigens derived from either<jats:italic>E. multilocularis<\/jats:italic>or<jats:italic>E. granulosus<\/jats:italic>. Therefore, parameters other than some minor differences in the primary sequence seem to be responsible for the lack of antigen II\/3 recognition in cystic echinococcosis.<\/jats:p><jats:p>Note<\/jats:p><jats:p>Nucleotide sequence data reported in this paper have been submitted to the GenBank<jats:sup>\u00ae<\/jats:sup>data base with the accession numbers U05573 and U05574.<\/jats:p>","DOI":"10.1017\/s0031182000076344","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T13:55:23Z","timestamp":1244210123000},"page":"223-232","source":"Crossref","is-referenced-by-count":27,"title":["Comparative analysis of full-length antigen II\/3 from<i>Echinococcus multilocularis<\/i>and<i>E. granulosus<\/i>"],"prefix":"10.1017","volume":"109","author":[{"given":"R.","family":"Felleisen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Gottstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000076344_ref015","doi-asserted-by":"publisher","DOI":"10.1007\/BF00927424"},{"key":"S0031182000076344_ref003","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000079300"},{"key":"S0031182000076344_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(91)90108-I"},{"key":"S0031182000076344_ref022","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(88)90162-4"},{"key":"S0031182000076344_ref007","doi-asserted-by":"publisher","DOI":"10.1021\/bi00709a025"},{"key":"S0031182000076344_ref009","doi-asserted-by":"publisher","DOI":"10.1007\/BF00937086"},{"key":"S0031182000076344_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(94)90130-9"},{"key":"S0031182000076344_ref001","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1990.43.506"},{"key":"S0031182000076344_ref002","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.1Part1.387"},{"key":"S0031182000076344_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(93)90052-Y"},{"key":"S0031182000076344_ref008","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.5.3.248"},{"key":"S0031182000076344_ref010","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1128\/JCM.31.2.373-376.1993","article-title":"Improved primary immunodiagnosis of alveolar echinococcosis (Echinococcus multilocularis infection of humans) by Em2plus-ELISA","volume":"31","author":"Gottstein","year":"1993","journal-title":"Journal of Clinical Microbiology"},{"key":"S0031182000076344_ref020","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"S0031182000076344_ref013","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"S0031182000076344_ref014","doi-asserted-by":"publisher","DOI":"10.1042\/bj2400001"},{"key":"S0031182000076344_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(89)90187-4"},{"key":"S0031182000076344_ref017","doi-asserted-by":"crossref","first-page":"11955","DOI":"10.1016\/S0021-9258(18)43211-5","article-title":"Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples","volume":"256","author":"Olmsted","year":"1981","journal-title":"Journal of Biological Chemistry"},{"key":"S0031182000076344_ref018","volume-title":"Molecular Cloning. A Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"S0031182000076344_ref019","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90005-4"},{"key":"S0031182000076344_ref021","doi-asserted-by":"publisher","DOI":"10.1126\/science.325648"},{"key":"S0031182000076344_ref011","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(91)90220-Z"},{"key":"S0031182000076344_ref004","first-page":"11","article-title":"Rapid amplification of cDNA ends (RACE): user friendly cDNA cloning","volume":"5","author":"Frohman","year":"1990","journal-title":"Amplifications"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000076344","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,6]],"date-time":"2021-10-06T23:04:35Z","timestamp":1633561475000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000076344\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,8]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,8]]}},"alternative-id":["S0031182000076344"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000076344","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,8]]}}}