{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T20:13:05Z","timestamp":1694635985469},"reference-count":17,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":6884,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1990,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Vesiculated protoscoleces (VP) were produced by culturing freshly collected protoscoleces from<jats:italic>Echinococcus granulosus<\/jats:italic>horse liver hydatids in RPMI 1640 monophasic medium at 37\u00b0C for 18 days. Half of the VP were used as such, the other half used after killing them by freeze\u2014thawing. Nine-day-old chicken heart fragments (CHF) were cultured in MEM at 37\u00b0C for 72 h. Subsequently, CHF were put together with live and dead VP, respectively, for up to 53 days, on a semisolid medium consisting of agar, Ringer's and MEM. Time-dependent histological observations revealed that dead VP were surrounded by CHF cells. Dead VP tissue was eventually internalized and disintegrated in about 1 week. Live VP penetrated into the CHF tissue and further developed into small hydatid cysts, located within the boundaries of the experimental \u2018host\u2019 tissue. The amorphous-looking contact region PAP-stained positively only with anti-<jats:italic>E. granulosus<\/jats:italic>serum and not with anti-CHF serum; it was considered identical to the normal laminated layer. The invasion of VP in CHF tissue proved to be different from a tumour or a bacterial invasion: it was concluded that the confrontation of VP and CHF had resulted in an \u2018<jats:italic>in vitro<\/jats:italic>cohabitation\u2019 rather than in an \u2018<jats:italic>in vitro<\/jats:italic>infecion\u2019.<\/jats:p>","DOI":"10.1017\/s0031182000078756","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:40:43Z","timestamp":1244212843000},"page":"453-458","source":"Crossref","is-referenced-by-count":1,"title":["Development of larval<i>Echinococcus granulosus<\/i>confronted with chicken heart tissue<i>in vitro<\/i>"],"prefix":"10.1017","volume":"100","author":[{"given":"D.","family":"Janssen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. H.","family":"De Rycke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"De Ridder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000078756_ref007","first-page":"410","article-title":"Echinococcus granulosus: cytotoxic effects of hydatid cyst fluid on intermediate host cells.","volume":"65","author":"Osuna","year":"1985","journal-title":"Annales de la Soci\u00e9t\u00e9 belge de M\u00e9decine tropicale"},{"key":"S0031182000078756_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(74)90057-5"},{"key":"S0031182000078756_ref016","first-page":"5162","article-title":"Invasiveness of hyperplastic nodule cells from diethylnitrosamine treated rat liver","volume":"41","author":"Wanson","year":"1981","journal-title":"Cancer Research"},{"key":"S0031182000078756_ref005","first-page":"143","volume-title":"The Biology of Echinococcus and Hydatid Diseases","author":"Howell","year":"1986"},{"key":"S0031182000078756_ref004","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(76)90083-7"},{"key":"S0031182000078756_ref002","unstructured":"De Wit M. (1987). Study on the occurrence of subspeciation in Echinococcus granulosus (hydatidosis) in Belgium [In Flemish: Onderzoek naar het voorkomen van subspeciatie van Echinococcus granulosus (hydatidosis) in Belgi\u00eb]. Thesis, State University of Gent, Belgium."},{"key":"S0031182000078756_ref010","volume-title":"Mikroskopische Technik","author":"Romeis","year":"1968"},{"key":"S0031182000078756_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(89)90210-X"},{"key":"S0031182000078756_ref013","doi-asserted-by":"publisher","DOI":"10.1038\/2111374a0"},{"key":"S0031182000078756_ref001","doi-asserted-by":"publisher","DOI":"10.1007\/BF00390374"},{"key":"S0031182000078756_ref017","doi-asserted-by":"publisher","DOI":"10.1086\/physzool.7.1.30151213"},{"key":"S0031182000078756_ref006","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/BF02889094","article-title":"Method of study of the invasion of malignant C3H mouse fibroblasts into embryonic chick heart in vitro","volume":"30","author":"Mareel","year":"1979","journal-title":"Virchows Archives B. Cell Pathology"},{"key":"S0031182000078756_ref008","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000050599"},{"key":"S0031182000078756_ref009","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000064143"},{"key":"S0031182000078756_ref011","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000027256"},{"key":"S0031182000078756_ref014","volume-title":"Immunocytochemistry","author":"Stenberger","year":"1974"},{"key":"S0031182000078756_ref015","first-page":"193","volume-title":"The Cultivation of Parasites in Vitro","author":"Voge","year":"1978"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000078756","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,6]],"date-time":"2021-10-06T23:13:28Z","timestamp":1633562008000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000078756\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,6]]}},"alternative-id":["S0031182000078756"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000078756","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6]]}}}