{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:23:12Z","timestamp":1792660992445,"version":"4.5.0"},"reference-count":13,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":6884,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1990,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>A size selected genomic DNA library was constructed using DNA extracted from <jats:italic>Taenia saginata<\/jats:italic>. The DNA was digested using the restriction enzyme <jats:italic>EcoR<\/jats:italic>1 under star conditions and the 2\u20134 kbase fraction, selected following sucrose density-gradient separation, was cloned in the bacteriophage \u03bbgt 10. A panel of cestode DNAs including <jats:italic>Taenia saginata, Taenia solium, Taenia taeniaeformis, Taenia crassiceps, Echinococcus granulosus<\/jats:italic> and DNAs of bovine, porcine and human origin were used in conjunction with hybridization analysis to identify two recombinant bacteriophages. The first probe, designated HDP1, reacted specifically with <jats:italic>T. saginata<\/jats:italic> DNA. The second, designated HDP2, reacted with DNA from both <jats:italic>T. saginata<\/jats:italic> and <jats:italic>T. solium<\/jats:italic> but not the other DNA samples and thus provided a general reagent for positive identification of fragments of <jats:italic>Taenia<\/jats:italic> spp. proglottides of human faecal origin. If used in conjunction the two DNA probes allow positive identification of <jats:italic>T. saginata<\/jats:italic>. In the clinical situation it is important to be able to distinguish <jats:italic>T. saginata<\/jats:italic> and <jats:italic>T. solium<\/jats:italic> infections and DNA probes such as these may be useful in such differentiation.<\/jats:p>","DOI":"10.1017\/s0031182000078768","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:40:43Z","timestamp":1244212843000},"page":"459-461","source":"Crossref","is-referenced-by-count":45,"title":["Differential diagnosis of <i>Taenia saginata<\/i> and <i>Taenia solium<\/i> with DNA probes"],"prefix":"10.1017","volume":"100","author":[{"given":"L. J. S.","family":"Harrison","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Delgado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. M. E.","family":"Parkhouse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000078768_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(77)90111-0"},{"key":"S0031182000078768_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(89)90114-X"},{"key":"S0031182000078768_ref007","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(78)90068-1"},{"key":"S0031182000078768_ref001","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000083463"},{"key":"S0031182000078768_ref012","doi-asserted-by":"publisher","DOI":"10.1017\/S003118200006683X"},{"key":"S0031182000078768_ref009","first-page":"234","article-title":"Identification of tapeworms","volume":"46","author":"Proctor","year":"1972","journal-title":"South African Medical Journal"},{"key":"S0031182000078768_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(77)90052-3"},{"key":"S0031182000078768_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(80)90044-2"},{"key":"S0031182000078768_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(88)90204-9"},{"key":"S0031182000078768_ref004","volume-title":"Cysticercosis: Present State of Knowledge and Perspectives","author":"Flisser","year":"1982"},{"key":"S0031182000078768_ref008","volume-title":"Molecular Cloning","author":"Maniatis","year":"1982"},{"key":"S0031182000078768_ref011","doi-asserted-by":"crossref","unstructured":"Rishi A. K. & McManus D. P. (1987). DNA probes which unambiguously distinguish Taenia solium from Taenia saginata. The Lancet, 1275\u20136.","DOI":"10.1016\/S0140-6736(87)91886-1"},{"key":"S0031182000078768_ref013","volume-title":"Helminths, Arthropods and Protozoa of Domesticated Animals","author":"Soulsby","year":"1982"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000078768","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,18]],"date-time":"2019-05-18T21:12:38Z","timestamp":1558213958000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000078768\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,6]]}},"alternative-id":["S0031182000078768"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000078768","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6]]}}}