{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T12:56:00Z","timestamp":1761310560139},"reference-count":16,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":5696,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1993,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>A recombinant<jats:italic>Echinococcus multilocularis<\/jats:italic>antigen (II\/3\u201310), which had previously been shown to exhibit immunodiagnostic characteristics highly specific for human alveolar echinococcosis, and the corresponding native parasite antigen, were further characterized with immunochemical and molecular biological methods. Immunoblot analysis using a polyclonal antiserum raised in rabbits against the recombinant protein, and subsequent Northern hybridization analysis, revealed that the native antigen was expressed by<jats:italic>E. multilocularis<\/jats:italic>at the adult as well as at the metacestode stage. In metacestodes, the antigen was shown by using indirect immunofluorescence and the same antiserum to be localized within the germinal layer and membrane structures of developing protoscolices. Electrophoretic analyses revealed remarkable differences in the apparent molecular weight of the antigen under reducing and non-reducing conditions. In further immunoblot analyses, anti-II\/3\u201310 antibodies identified the corresponding epitopes on bands with identical<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>in all<jats:italic>E. multilocularis<\/jats:italic>isolates investigated (European, Asian and North American). By Southern hybridization analyses of the respective gene, phylogenetically related sequences were shown to be present in other helminth species such as<jats:italic>E. granulosus<\/jats:italic>and several<jats:italic>Taenia<\/jats:italic>spp. In the same respect, immunoblotting revealed that anti-II\/3\u201310 antibodies reacted with antigens of different<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>from various<jats:italic>E. granulosus<\/jats:italic>isolates and some other cestode species, indicating the presence of shared and thus potentially cross-reacting epitopes. The relevance of these findings for the immunodiagnostic performance of the recombinant antigen is discussed.<\/jats:p>","DOI":"10.1017\/s0031182000079300","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T14:08:14Z","timestamp":1244210894000},"page":"335-342","source":"Crossref","is-referenced-by-count":34,"title":["<i>Echinococcus multilocularis<\/i>: molecular and immunochemical characterization of diagnostic antigen II\/3-10"],"prefix":"10.1017","volume":"107","author":[{"given":"R.","family":"Felleisen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Gottstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000079300_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(88)90162-4"},{"key":"S0031182000079300_ref015","doi-asserted-by":"publisher","DOI":"10.1126\/science.325648"},{"key":"S0031182000079300_ref014","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"S0031182000079300_ref013","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"S0031182000079300_ref011","doi-asserted-by":"publisher","DOI":"10.1021\/bi00640a033"},{"key":"S0031182000079300_ref010","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"S0031182000079300_ref008","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1128\/JCM.31.2.373-376.1993","article-title":"Improved primary immunodiagnosis of alveolar echinococcosis in humans by an enzyme- linked immunosorbent assay using the Em2plus-antigen","volume":"31","author":"Gottstein","year":"1993","journal-title":"Journal of Clinical Microbiology"},{"key":"S0031182000079300_ref005","doi-asserted-by":"publisher","DOI":"10.1007\/BF00930916"},{"key":"S0031182000079300_ref004","doi-asserted-by":"publisher","DOI":"10.1021\/bi00709a025"},{"key":"S0031182000079300_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(91)90108-I"},{"key":"S0031182000079300_ref002","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/0003-2697(84)90381-6","article-title":"Addendum: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity","volume":"137","author":"Feinberg","year":"1984","journal-title":"Analytical Biochemistry"},{"key":"S0031182000079300_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90418-9"},{"key":"S0031182000079300_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(89)90187-4"},{"key":"S0031182000079300_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(91)90004-P"},{"key":"S0031182000079300_ref006","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1991.tb00560.x"},{"key":"S0031182000079300_ref007","doi-asserted-by":"publisher","DOI":"10.1007\/BF00937086"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000079300","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T06:44:40Z","timestamp":1589870680000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000079300\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,9]]},"references-count":16,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1993,9]]}},"alternative-id":["S0031182000079300"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000079300","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,9]]}}}