{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T08:31:39Z","timestamp":1697963499498},"reference-count":31,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":8162,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1986,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Adult<jats:italic>Wuchereria bancrofti<\/jats:italic>were recovered from infected<jats:italic>Presbytis cristatus<\/jats:italic>monkeys and radio-isotope labelled extrinsically with<jats:sup>125<\/jats:sup>I and<jats:italic>in vitro<\/jats:italic>with [<jats:sup>35<\/jats:sup>S]methionine.<jats:sup>125<\/jats:sup>I labelling of the surface of adult<jats:italic>W. bancrofti<\/jats:italic>permitted a comparison between the major surface antigens of this species and those from the related lymphatic filariae,<jats:italic>Brugia malayi<\/jats:italic>and<jats:italic>B. pahangi<\/jats:italic>. All species bear a prominent<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>29 000 surface antigen but among the differences observed were the strongly labelled molecules with<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>58 000 and 67 000 in<jats:italic>W. bancrofti<\/jats:italic>which are extremely faint in the<jats:italic>Brugia<\/jats:italic>species. The [<jats:sup>35<\/jats:sup>S]methionine label was effectively incorporated into somatic parasite proteins<jats:italic>in vitro<\/jats:italic>although it was not possible to identify any secreted proteins in this way. The antigenicity of these products was investigated using a variety of sera from homologous and heterologous infections and the immunoprecipitation patterns highlighted particular differences between somatic proteins of male and female worms. One secreted antigen was detected, however, by virtue of its phosphorylcholine epitopes, in the culture medium of mixed adult worms; medium from male<jats:italic>W. bancrofti<\/jats:italic>adults was negative although homogenates of either sex of adult<jats:italic>W. bancrofti<\/jats:italic>were strongly positive in the same system.<\/jats:p>","DOI":"10.1017\/s0031182000081269","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:17:13Z","timestamp":1244218633000},"page":"559-569","source":"Crossref","is-referenced-by-count":16,"title":["Antigenic characterization of adult<i>Wuchereria bancrofti<\/i>filarial nematodes"],"prefix":"10.1017","volume":"93","author":[{"given":"T. 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