{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:05:53Z","timestamp":1762160753129},"reference-count":16,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":8162,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1986,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Previously we reported the isolation of several<jats:italic>Escherichia coli<\/jats:italic>clones expressing fragments of<jats:italic>Taenia taeniaeformis<\/jats:italic>antigens as \u03b2-galactosidase fused proteins (Bowtell, Saint, Rickard &amp; Mitchell, 1984). Here we describe the isolation of additional antigen-expressing clones from a larval cDNA library and the assignment of these clones to 7 antigen families. These were isolated with a polyspecific rabbit antiserum raised to the oncosphere. Since this serum was capable of reacting with a large number of antigens, it was important to develop techniques for rapidly determining the identity of the native<jats:italic>T. taeniaeformis<\/jats:italic>molecule corresponding to a cloned antigen gene. These included active immunization of rabbits with fused proteins and several techniques involving affinity purification on immobilized fused proteins. The reactivity of the antigen-positive clones with sera from humans infected with related parasites was also assessed. Finally, immunization of mice with several fused proteins failed to protect against subsequent infection, although antigens previously identified as candidate host-protective antigens (Bowtell, Mitchell, Anders, Lightowlers &amp; Rickard, 1983) have yet to be identified in the expression library.<\/jats:p>","DOI":"10.1017\/s0031182000081300","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:17:13Z","timestamp":1244218633000},"page":"599-610","source":"Crossref","is-referenced-by-count":11,"title":["Immunochemical analysis of<i>Taenia taeniaeformis<\/i>antigens expressed in<i>Escherichia coli<\/i>"],"prefix":"10.1017","volume":"93","author":[{"given":"D. D. L.","family":"Bowtell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. B.","family":"Saint","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. D.","family":"Rickard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. F.","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000081300_ref004","doi-asserted-by":"publisher","DOI":"10.1038\/310789a0"},{"key":"S0031182000081300_ref014","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(86)90090-3"},{"key":"S0031182000081300_ref007","first-page":"437","volume-title":"Cysticercosis: Present State of Knowledge and Perspectives","author":"Guerra","year":"1982"},{"key":"S0031182000081300_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0735-0651(84)90049-9"},{"key":"S0031182000081300_ref005","unstructured":"Gill A. C. (1986). Characteristics of a vaccine strain of Babesia bovis. Ph.D. thesis, University of Melbourne."},{"key":"S0031182000081300_ref012","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4049\/jimmunol.136.5.1712","article-title":"Isolation and partial characterization of an eosinophil chemotactic factor from metacestode of Taenia taeniaeformis (ECF-Tt)","volume":"136","author":"Potter","year":"1986","journal-title":"Journal of Immunology"},{"key":"S0031182000081300_ref016","doi-asserted-by":"publisher","DOI":"10.1126\/science.6356359"},{"key":"S0031182000081300_ref013","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.4049\/jimmunol.115.5.1459","article-title":"Isolation of the most immunoreactive antigens of Echinococcus granulosus from sheep hydatid fluid","volume":"115","author":"Pozzuoli","year":"1975","journal-title":"Journal of Immunology"},{"key":"S0031182000081300_ref010","first-page":"S83","volume-title":"Parasites and Molecular Biology: Applications of New Techniques. Symposia of the British Society for Parasitology","volume":"23","author":"Kemp","year":"1986"},{"key":"S0031182000081300_ref011","first-page":"481","article-title":"A mechanism to account for mouse strain variation in resistance to the larval cestode, Taenia taeniaeformis","volume":"39","author":"Mitchell","year":"1980","journal-title":"Immunology"},{"key":"S0031182000081300_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(83)90087-5"},{"key":"S0031182000081300_ref015","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.5.1194"},{"key":"S0031182000081300_ref001","first-page":"177","article-title":"Plasmodium falciparum cDNA clones expressed in Escherichia coli encode many distinctive antigens","volume":"2","author":"Anders","year":"1984","journal-title":"Molecular Biology in Medicine"},{"key":"S0031182000081300_ref006","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.10.3961"},{"key":"S0031182000081300_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(84)90111-7"},{"key":"S0031182000081300_ref009","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.12.3787"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000081300","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T14:37:21Z","timestamp":1685025441000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000081300\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,12]]},"references-count":16,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1986,12]]}},"alternative-id":["S0031182000081300"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000081300","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,12]]}}}