{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T12:56:01Z","timestamp":1761310561160},"reference-count":19,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":7735,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1988,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Antigenicity of adult <jats:italic>Opisthorchis viverrini<\/jats:italic>, metacercariae and eggs was investigated by radio-immunoprecipitation and polyacrylamide gel electrophoresis using sera from patients with opisthorchiasis and from hamsters experimentally infected with <jats:italic>0. viverrini<\/jats:italic> metacercariae. <jats:sup>125<\/jats:sup>I-labelled aqueous somatic extract and <jats:italic>in vitro<\/jats:italic> metabolic products of adult worms, and the extracts of metacercariae and eggs were immunoprecipitated, electrophoresed and autoradiographed and major antigens were characterized. Although components with <jats:italic>M<jats:sub>r<\/jats:sub><\/jats:italic> of &gt; 116, 89, 78 and 20 kD appeared to be specifically associated with the somatic extract of adult fluke, the 89 kD protein was the most prominent component found in the <jats:italic>in vitro<\/jats:italic> culture fluid of adult worms and the metacercarial extract. Hamsters, experimentally infected with <jats:italic>0. viverrini<\/jats:italic>, also showed the presence of antibody reactive with the 89 kD component within 15 days of infection. The potential of this antigen in immunodiagnosis is discussed.<\/jats:p>","DOI":"10.1017\/s0031182000081701","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:11:33Z","timestamp":1244218293000},"page":"119-128","source":"Crossref","is-referenced-by-count":33,"title":["Analysis of <i>Opisthorchis viverrini<\/i> antigens by immunoprecipitation and polyacrylamide gel electrophoresis"],"prefix":"10.1017","volume":"96","author":[{"given":"S.","family":"Wongratanacheewin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Chawengkirttikul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Bunnag","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Sirisinha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000081701_ref011","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00025943"},{"key":"S0031182000081701_ref001","first-page":"135","article-title":"Detection of antibodies in sera from patients with opisthorchiasis","volume":"19","author":"Boonpucknavig","year":"1986","journal-title":"Journal of Clinical and Laboratory 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