{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T13:19:52Z","timestamp":1697807992204},"reference-count":24,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2009,4,6]],"date-time":"2009-04-06T00:00:00Z","timestamp":1238976000000},"content-version":"unspecified","delay-in-days":9258,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1983,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Experimental procedures are described that lead to the formation of sealed red cell ghosts capable of efficient invasion by malaria parasites. Both human and monkey cells have been studied with respect to invasion by<jats:italic>Plasmodium falciparum<\/jats:italic>and<jats:italic>P. knowlesi<\/jats:italic>respectively. Resealed ghosts containing about half the normal concentration of haemoglobin are prepared by osmotic lysis and resealing at a haematocrit of 70%. When examined in the scanning electron microscope, populations of these ghosts contain few echinocytes, but have an abundance of stomatocytic forms. When undiluted haemolysate is substituted for the aqueous saline diluent, in which the cells are suspended for lysis and resealing, discocytes result, with a morphology very similar to that of the original cells. Invasion is somewhat, but not dramatically higher for this material. An investigation of the effect of intracellular potassium concentration revealed that this had no major effect on invasion. A small increase in invasion resulted from addition of 50 or 100 mg\/ml of albumin to the medium in which the cells were resealed, but intra-erythrocytic protein content<jats:italic>per se<\/jats:italic>is evidently not a major factor in determining the efficiency of invasion. Augmentation of the cytoplasmic ATP concentration during lysis and resealing increased the level of parasitaemia significantly, and the parasites in these ghosts developed normally and gave rise to viable merozoites. It was found that albumin at certain concentrations passed freely into the lysed cells and reached equilibrium with that in the external medium. By contrast, the high molecular weight solute Blue Dextran 2000 was completely excluded from the lysed cells.<\/jats:p>","DOI":"10.1017\/s0031182000082950","type":"journal-article","created":{"date-parts":[[2009,6,5]],"date-time":"2009-06-05T16:32:01Z","timestamp":1244219521000},"page":"429-438","source":"Crossref","is-referenced-by-count":12,"title":["Properties of red cell ghost preparations susceptible to invasion by malaria parasites"],"prefix":"10.1017","volume":"87","author":[{"given":"A. R.","family":"Dluzewski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Rangachari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J. M.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. B.","family":"Gratzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2009,4,6]]},"reference":[{"key":"S0031182000082950_ref012","doi-asserted-by":"publisher","DOI":"10.1084\/jem.149.1.172"},{"key":"S0031182000082950_ref010","doi-asserted-by":"crossref","first-page":"11660","DOI":"10.1016\/S0021-9258(18)33813-4","article-title":"Dynamics of the holes in human erythrocyte membrane ghosts","volume":"257","author":"Lieber","year":"1982","journal-title":"Journal of Biological Chemistry"},{"key":"S0031182000082950_ref023","doi-asserted-by":"publisher","DOI":"10.1126\/science.781840"},{"key":"S0031182000082950_ref014","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.95.3.757"},{"key":"S0031182000082950_ref001","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-88062-9"},{"key":"S0031182000082950_ref019","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.73.3.638"},{"key":"S0031182000082950_ref017","first-page":"755","article-title":"Purification of mature schizonts of Plasmodium falciparum on colloidal silica gradients","volume":"60","author":"Saul","year":"1982","journal-title":"Bulletin of the World Health Organization"},{"key":"S0031182000082950_ref024","first-page":"179","article-title":"Invasion and growth of Plasmodium falciparum in different types of human erythrocyte","volume":"55","author":"Wilson","year":"1977","journal-title":"Bulletin of the World Health Organization"},{"key":"S0031182000082950_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(79)90009-2"},{"key":"S0031182000082950_ref002","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(63)90042-0"},{"key":"S0031182000082950_ref003","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1981.tb07201.x"},{"key":"S0031182000082950_ref004","first-page":"636","article-title":"The effect of ATP on the entry of malaria parasites into resealed ghosts of human erythrocytes","volume":"29","author":"Dluzewski","year":"1982","journal-title":"Journal of Protozoology"},{"key":"S0031182000082950_ref006","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1983.tb02845.x"},{"key":"S0031182000082950_ref007","doi-asserted-by":"publisher","DOI":"10.1042\/bj0570626"},{"key":"S0031182000082950_ref009","doi-asserted-by":"crossref","first-page":"11651","DOI":"10.1016\/S0021-9258(18)33812-2","article-title":"A description of the holes in human erythrocyte membrane ghosts","volume":"257","author":"Lieber","year":"1982","journal-title":"Journal of Biological Chemistry"},{"key":"S0031182000082950_ref011","first-page":"157","article-title":"Hypothesis on the mechanism of erythrocyte invasion by malaria merozoites","volume":"55","author":"Miller","year":"1977","journal-title":"Bulletin of the World Health Organization"},{"key":"S0031182000082950_ref013","first-page":"537","volume-title":"The Red Cell, Fifth Ann Arbor Conference","author":"Olson","year":"1981"},{"key":"S0031182000082950_ref015","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.bmb.a071749"},{"key":"S0031182000082950_ref018","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.4.1740"},{"key":"S0031182000082950_ref020","doi-asserted-by":"publisher","DOI":"10.1007\/BF01851280"},{"key":"S0031182000082950_ref021","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(79)90585-0"},{"key":"S0031182000082950_ref022","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1971.tb03341.x"},{"key":"S0031182000082950_ref005","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(83)90106-8"},{"key":"S0031182000082950_ref016","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1978.11719283"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000082950","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,6]],"date-time":"2021-10-06T23:47:17Z","timestamp":1633564037000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000082950\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,12]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1983,12]]}},"alternative-id":["S0031182000082950"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000082950","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,12]]}}}