{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T04:37:25Z","timestamp":1759639045069},"reference-count":25,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2011,11,8]],"date-time":"2011-11-08T00:00:00Z","timestamp":1320710400000},"content-version":"unspecified","delay-in-days":10995,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1981,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Mice experimentally infected with the hydatid parasite <jats:italic>Echinococcus granulosus<\/jats:italic> (secondary echinococcosis), were used as spleen cell donors for hybridoma production. Two hybridomas were produced which secreted antibody with anti-<jats:italic>E. granulosus<\/jats:italic> cyst fluid (EgCF) activity. Radio-immunoassays comparing the binding of these hybridoma-derived antibodies to <jats:italic>E. granulosus<\/jats:italic> versus several other antigen preparations from sheep parasites showed that the antibodies had a high degree of specificity for the hydatid parasite. However, using a panel of clinically defined sheep sera in a competitive radio-immunoassay, the binding of the hybridoma-derived antibodies to EgCF was inhibited strongly by sera from sheep infected with the common parasites <jats:italic>Fasciola hepatica<\/jats:italic> and <jats:italic>Taenia hydatigena<\/jats:italic>. When the hybridoma-derived antibodies were conjugated to CNBr-activated Sepharose and the crude EgCF applied to the affinity column, the \u2018run through\u2019 antigen preparation showed a significant increase in specificity for experimental <jats:italic>E. granulosus<\/jats:italic> infection. A useful means of applying the hybridoma technology in the development of new immunodiagnostic reagents may be to select those murine hybridomas which produce high-affinity, cross-reacting antibodies and to use these antibodies in the processing of homologous antigen by affinity chromatography.<\/jats:p>","DOI":"10.1017\/s0031182000085310","type":"journal-article","created":{"date-parts":[[2011,11,8]],"date-time":"2011-11-08T14:46:15Z","timestamp":1320763575000},"page":"303-317","source":"Crossref","is-referenced-by-count":23,"title":["Murine hybridoma-derived antibodies in the processing of antigens for the immunodiagnosis of hydatid (<i>Echinococcus granulosus<\/i>) infection in sheep"],"prefix":"10.1017","volume":"83","author":[{"given":"P. S.","family":"Craig","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. E.","family":"Hocking","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. F.","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. D.","family":"Rickard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2011,11,8]]},"reference":[{"key":"S0031182000085310_ref024","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(78)90209-2"},{"key":"S0031182000085310_ref021","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000027256"},{"key":"S0031182000085310_ref020","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1979.tb15241.x"},{"key":"S0031182000085310_ref014","first-page":"39","article-title":"Sequential affinity chromatography for the purification of antigens extracted from Onchocerca volvulus adult worms","volume":"29","author":"Marcoullis","year":"1978","journal-title":"Zeitschrift f\u00fcr Tropenmedizin und Parasitologie"},{"key":"S0031182000085310_ref010","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000078252"},{"key":"S0031182000085310_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(72)90094-2"},{"key":"S0031182000085310_ref011","first-page":"25","article-title":"A review of serological tests for the diagnosis of hydatid disease","volume":"39","author":"Kagan","year":"1968","journal-title":"Bulletin of the World Health Organization"},{"key":"S0031182000085310_ref017","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1979.32"},{"key":"S0031182000085310_ref001","doi-asserted-by":"publisher","DOI":"10.1007\/BF00380555"},{"key":"S0031182000085310_ref022","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(75)90021-1"},{"key":"S0031182000085310_ref008","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830071018"},{"key":"S0031182000085310_ref019","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1969.18.7"},{"key":"S0031182000085310_ref007","doi-asserted-by":"publisher","DOI":"10.1038\/266550a0"},{"key":"S0031182000085310_ref013","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830060713"},{"key":"S0031182000085310_ref006","doi-asserted-by":"publisher","DOI":"10.1007\/BF00925520"},{"key":"S0031182000085310_ref003","first-page":"27","article-title":"Le diagnostic immunoelectrophor\u00e9tique de l'hydatidose","author":"Capron","year":"1967","journal-title":"In 8IMEP Ed. (Lyon)"},{"key":"S0031182000085310_ref004","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1980.33"},{"key":"S0031182000085310_ref016","first-page":"499","article-title":"Hydatidosis: a global problem of increasing importance","volume":"55","author":"Matossian","year":"1977","journal-title":"Bulletin of the World Health Organization"},{"key":"S0031182000085310_ref018","doi-asserted-by":"publisher","DOI":"10.1159\/000231669"},{"key":"S0031182000085310_ref002","doi-asserted-by":"publisher","DOI":"10.1084\/jem.151.6.1436"},{"key":"S0031182000085310_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(77)90070-0"},{"key":"S0031182000085310_ref023","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0034-5288(18)33156-4","article-title":"Comparative antigenic characterization of Echinococcus granulosus and Taenia hydatigena cyst fluids by immunoelectrophoresis","volume":"23","author":"Varela-Diaz","year":"1977","journal-title":"Research in Veterinary Science"},{"key":"S0031182000085310_ref005","doi-asserted-by":"publisher","DOI":"10.1007\/BF00930493"},{"key":"S0031182000085310_ref025","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1979.tb00693.x"},{"key":"S0031182000085310_ref012","doi-asserted-by":"publisher","DOI":"10.1038\/256495a0"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182000085310","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,25]],"date-time":"2019-05-25T19:31:04Z","timestamp":1558812664000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182000085310\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,10]]},"references-count":25,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1981,10]]}},"alternative-id":["S0031182000085310"],"URL":"https:\/\/doi.org\/10.1017\/s0031182000085310","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,10]]}}}