{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T08:50:13Z","timestamp":1762159813562,"version":"3.41.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2002,5,21]],"date-time":"2002-05-21T00:00:00Z","timestamp":1021939200000},"content-version":"unspecified","delay-in-days":474,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:p>In this paper we report on the biochemical presence of nitric oxide synthase (NOS)-like activity in <jats:italic>Ascaris suum <\/jats:italic>tissue and examine the pharmacological effect of NO donors on <jats:italic>A. suum <\/jats:italic>muscle strip preparation. NOS activity was determined by monitoring the formation of [<jats:sup>3<\/jats:sup>H]citrulline from [<jats:sup>3<\/jats:sup>H]<jats:sc>L<\/jats:sc>-arginine and NO formation via the oxyhaemoglobin assay. Neuromuscular tissue from <jats:italic>A. suum<\/jats:italic> which stained positively for NADPH diaphorase, contained NOS activity. Neither NOS activity nor NADPH diaphorase staining was detected in intestinal tissue. The absence of Ca<jats:sup>2+<\/jats:sup>, NADPH and other co-factors normally required for mammalian neuronal NOS activity only partially reduced the formation of both citrulline and NO by <jats:italic>A. suum<\/jats:italic> neuromuscular homogenate. The results of the biochemical assays indicate the presence of an enzyme capable of producing NO and citrulline, but with a different profile from that of rat neuronal NOS. We also present preliminary evidence for the action of NO (NO donors) in the neuromuscular system of <jats:italic>A. suum<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s003118200100720x","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:39:16Z","timestamp":1027777156000},"page":"219-231","source":"Crossref","is-referenced-by-count":17,"title":["Characterization of a putative nitric oxide synthase in the neuromuscular system of the parasitic nematode, <i>Ascaris suum<\/i>"],"prefix":"10.1017","volume":"122","author":[{"given":"Z.A.","family":"BASCAL","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.M.","family":"CUNNINGHAM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"HOLDEN-DYE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"O'SHEA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.J.","family":"WALKER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2002,5,21]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S003118200100720X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,21]],"date-time":"2025-06-21T05:18:22Z","timestamp":1750483102000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S003118200100720X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,2]]}},"alternative-id":["S003118200100720X"],"URL":"https:\/\/doi.org\/10.1017\/s003118200100720x","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"type":"print","value":"0031-1820"},{"type":"electronic","value":"1469-8161"}],"subject":[],"published":{"date-parts":[[2001,2]]}}}