{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T03:49:06Z","timestamp":1791085746101,"version":"4.1.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2003,2,6]],"date-time":"2003-02-06T00:00:00Z","timestamp":1044489600000},"content-version":"unspecified","delay-in-days":462,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2001,11]]},"abstract":"<jats:p><jats:italic>Leishmania (Leishmania) infantum<\/jats:italic> zymodeme MON-1 is responsible for the majority of visceral leishmaniasis cases around the Mediterranean basin, albeit that it causes also cutaneous forms. The MON classification is based on starch gel multilocus enzyme electrophoresis (MLEE) typing. The aim of this work was to explore further the genetic diversity and phylogenetic status of this zymodeme by alternative typing techniques. Fourteen <jats:italic>L<\/jats:italic>. (<jats:italic>L<\/jats:italic>.) <jats:italic>infantum<\/jats:italic>\/<jats:italic>L<\/jats:italic>. (<jats:italic>L<\/jats:italic>.) <jats:italic>chagasi<\/jats:italic> stocks identified as MON-1 by MLEE in reference laboratories, 3 <jats:italic>L. infantum<\/jats:italic> stocks attributed to other zymodemes (MON-24, MON-29, MON-33) and reference standard stocks belonging to other species (<jats:italic>L<\/jats:italic>. (<jats:italic>L<\/jats:italic>.) <jats:italic>major<\/jats:italic>, <jats:italic>L<\/jats:italic>. (<jats:italic>L<\/jats:italic>.) <jats:italic>tropica<\/jats:italic> and <jats:italic>L<\/jats:italic>. (<jats:italic>L<\/jats:italic>.) <jats:italic>donovani<\/jats:italic>) were characterized by 2 different markers: MLEE on cellulose acetate plates and Random Amplified Polymorphic DNA (RAPD). We have obtained 10 different genotypes with RAPD and 6 different genotypes with MLEE on cellulose acetate plates for the 14 <jats:italic>L. infantum\/L. chagasi<\/jats:italic> MON-1 stocks studied. MLEE and RAPD data gave quite congruent phylogenetic results: <jats:italic>L. infantum<\/jats:italic> zymodeme MON-1 was shown to be polyphyletic and genetically heterogeneous. This work confirms the necessity of using different markers to build up a robust phylogeny. Finally the epidemiological and clinical implications of these results are discussed.<\/jats:p>","DOI":"10.1017\/s003118200100871x","type":"journal-article","created":{"date-parts":[[2003,10,16]],"date-time":"2003-10-16T10:50:57Z","timestamp":1066301457000},"page":"425-432","source":"Crossref","is-referenced-by-count":39,"title":["Genetic heterogeneity and phylogenetic status of <i>Leishmania (Leishmania) infantum<\/i> zymodeme MON-1: epidemiological implications"],"prefix":"10.1017","volume":"123","author":[{"given":"M.","family":"HIDE","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. L.","family":"BA\u00d1ULS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"TIBAYRENC","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2003,2,6]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S003118200100871X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T22:43:37Z","timestamp":1557269017000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S003118200100871X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,11]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2001,11]]}},"alternative-id":["S003118200100871X"],"URL":"https:\/\/doi.org\/10.1017\/s003118200100871x","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,11]]}}}