{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T19:44:02Z","timestamp":1648583042101},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2003,1,17]],"date-time":"2003-01-17T00:00:00Z","timestamp":1042761600000},"content-version":"unspecified","delay-in-days":261,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2002,5]]},"abstract":"<jats:p><jats:italic>Fasciola hepatica<\/jats:italic>, a parasitic flatworm belonging to the Class Trematoda, is one of the first metazoan groups to possess a centralized nervous system. However, the electrophysiological properties of neurones in <jats:italic>F. hepatica<\/jats:italic> are largely unknown. In the present study, we acutely isolated viable neurones from <jats:italic>F. hepatica<\/jats:italic> and characterized their electrophysiological properties. A hyperpolarization-activated cation current was recorded in the cells using the whole-cell patch-clamp. The current was found to be activated slowly at membrane potentials negative to 0\u00a0mV and did not display any time-dependent inactivation. This current was reduced by 1\u00a0m<jats:sc>M<\/jats:sc> Gd<jats:sup>3+<\/jats:sup> to the level of the leak current, while 3\u00a0m<jats:sc>M<\/jats:sc> of Cs<jats:sup>+<\/jats:sup> had no effect. However, the current was inhibited by extracellular acidosis in the pH range 7.0\u20137.8, and the membrane potentials of these cells were depolarized by extracellular alkalosis in the pH range of 5.8 to 8.2. Gd<jats:sup>3+<\/jats:sup> (1\u00a0m<jats:sc>M<\/jats:sc>), which inhibited the pH-sensitive hyperpolarization-activated cation current, also hyperpolarized the cells. In summary, we isolated single neurones from <jats:italic>F. hepatica<\/jats:italic>, and these were found to express a pH-sensitive hyperpolarization-activated cation current. This current may participate in the membrane depolarization of <jats:italic>F. hepatica<\/jats:italic> neurones during alkaline challenge.<\/jats:p>","DOI":"10.1017\/s0031182002002299","type":"journal-article","created":{"date-parts":[[2003,2,5]],"date-time":"2003-02-05T16:08:19Z","timestamp":1044461299000},"page":"423-430","source":"Crossref","is-referenced-by-count":5,"title":["A gadolinium and pH-sensitive hyperpolarization-activated cation current in acutely isolated single neurones from <i>Fasciola hepatica<\/i>"],"prefix":"10.1017","volume":"125","author":[{"given":"H.\u00a0S.","family":"KIM","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.\u00a0Y.","family":"KAM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.\u00a0D.","family":"RYU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.\u00a0J.","family":"HONG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.\u00a0S.","family":"JEON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.\u00a0H.","family":"JEON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.\u00a0J.","family":"KIM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.\u00a0B.","family":"PARK","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2003,1,17]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182002002299","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T20:00:37Z","timestamp":1554148837000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182002002299\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2002,5]]}},"alternative-id":["S0031182002002299"],"URL":"https:\/\/doi.org\/10.1017\/s0031182002002299","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,5]]}}}