{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T02:49:09Z","timestamp":1773802149912,"version":"3.50.1"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2003,2,17]],"date-time":"2003-02-17T00:00:00Z","timestamp":1045440000000},"content-version":"unspecified","delay-in-days":47,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:p>A PCR procedure for the detection of <jats:italic>Opisthorchis viverrini<\/jats:italic> in experimentally infected bithynid snails and cyprinoid fishes was developed. This procedure was based on primers designed from a pOV-A6 specific probe sequence giving a 330 base-pair product. The detection was accomplished in host tissue homogenates to which a single cercaria or metacercaria was introduced. PCR can detect as little as a single cercaria artificially inoculated in a snail or a single metacercaria artificially inoculated in a fish sample. The method gave a 100% positivity rate for all infected snails or fishes. The method did not yield a 330 base-pair amplified product with other digenean fluke DNAs such as <jats:italic>Haplorchis taichui<\/jats:italic>, <jats:italic>Centrocestus<\/jats:italic> spp., <jats:italic>Echinostoma malayanum<\/jats:italic>, <jats:italic>Fasciola gigantica<\/jats:italic>, animal schistosomes, <jats:italic>Paragonimus heterotremus<\/jats:italic> or <jats:italic>Haplorchoides<\/jats:italic> spp. The assay has great potential for application in epidemiological surveys of both snail and fish intermediate hosts as well as for investigation of foodborne parasites in freshwater fishes.<\/jats:p>","DOI":"10.1017\/s0031182002002573","type":"journal-article","created":{"date-parts":[[2003,3,3]],"date-time":"2003-03-03T06:10:36Z","timestamp":1046671836000},"page":"63-67","source":"Crossref","is-referenced-by-count":31,"title":["Detection of <i>Opisthorchis viverrini<\/i> in experimentally infected bithynid snails and cyprinoid fishes by a PCR-based method"],"prefix":"10.1017","volume":"126","author":[{"given":"W.","family":"MALEEWONG","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. M.","family":"INTAPAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"WONGKHAM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"WONGSAROJ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"KOWSUWAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"PUMIDONMING","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"PONGSASKULCHOTI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"KITIKOON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2003,2,17]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182002002573","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T18:48:52Z","timestamp":1554835732000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182002002573\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["S0031182002002573"],"URL":"https:\/\/doi.org\/10.1017\/s0031182002002573","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1]]}}}